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[QUOTE=Unregistered;294672][b][u]NAME [/u][/b]: Seema Narasanagi [b][u]Email address [/u][/b]: seema.n062 AT gmail.com [b][u]OBJECTIVE [/u][/b]: Seeking a challenging position to use my skills to the optimum level and at the same time get an opportunity to enhance my knowledge in the field of Biochemistry. [b][u]BASIC QUALIFICATION [/u][/b]: June/July 2013 Master of Science (MSc) Biochemistry. University of Mysore. Mysore. (1st sem 8.08, 2nd sem 7.96, 3rd sem 8.05 and 4th 9.0 ) 84.29 % April/May 2011 BSc ( Biochemistry, Botany, Microbiology) Maharani’s Science College. J L B road Mysore. 88.36 % April/May 2008 Pre University Course (PCB) Kendriya Yidyalaya Kapurthala Cantt, Punjab. 76.0% March/April 2006 SSLC ( 10th standard ) Army School Kapurthala, Punjab. 70.0% [b][u]ACADEMIC PROJECT [/u][/b]: [b][u]Title [/u][/b]: “Oxidized LDL and oxidized phosphatidylcholine in atherosclerosis”. Under the guidance of Dr. Cletus J M D’Souza, Professor, Department of Studies in Biochemistry, University of Mysore, Manasagangotri. Mysore – 570006. [b][u]Aim and scope of the project work [/u][/b]: Since the phospholipids and fatty acids play a major role in atherosclerosis, the main objective of this study was to characterized the changes taking place in the fatty acid composition of oxidized LDL. The fatty acid composition of phospholipids is greatly influenced by the dietary fatty acids. Hence we wanted to characterized the nature of fatty acids in the LDL particle and see what happens to these fatty acids on oxidation with Cu++ ions. [b][u]Objective of the study [/u][/b]: [b][u]The overall objective of the study was to study the changes taking place in the lipids and proteins of LDL particle on oxidation ,specific objective are [/u][/b]: 1. To prepare LDL from human serum by ultracentrifugation. 2. To oxidize LDL by Cu++ ions and to characterize the changes in proteins and lipids. 3. To standardize a simple assay for PLA2 and to test the stimulation of endogenous PLA2 by oxidation. [b][u]DISSERTATION [/u][/b]: [b][u]Title [/u][/b]: NASH (Non alcoholic steato-hepatitis) Non-alcoholic steatohepatitis (NASH) is the most extreme form of NAFLD which is regarded as the major cause of cirrhosis of the liver. It is a condition that cause inflammation and accumulation of fat and fibrous tissue in the liver. NASH occurs in those who drink little to no alcohol. NASH has been observed for serious liver destruction and enhanced liver mortality. It has systemic consequences concerning insulin resistance, metabolic complications and cardiovascular diseases. NASH is seen more often in women than men. [b][u]COURSE WORK [/u][/b]: Venue : Department of Biochemistry. Biochemical techniques Biomolecules Concepts in Biometry and Communication Clinical Biochemistry Molecular biology Essentials of Microbiology Immunotechnology Membrane Biology Physico-chemical aspects of Biology Enzymology Carbohydrate and Lipid metabolism Proteins and Nucleic acid metabolism Genomics and Gene regulation Genetic Engineering and Biotechnology Biostatistics and research methodology Behavioral biology and ecology hormones and Cell signaling Physiology and Nutrition Plant Biochemistry and Bioenergetics Biostatistics & Bioinformatics [b][u]TECHNICAL SKILLS [/u][/b]: ** Basic microbiology and tissue culture techniques ** Colorimetric and titrimetric estimation of Biomolecules. ** Enzyme assays and kinetic analysis ** Chromatographic techniques - Paper Chromatography, Thin Layer Chromatography, Gel Filtration Chromatography, Ion Exchange Chromatography. ** Electrophoretic techniques - Acidic and Basic PAGE, SDS PAGE (Reducing and Non-reducing), Zymogram. ** Determination of snake venom enzyme activities – Protease, Phospholipase A2. ** Molecular techniques – Media preparation and culturing of E.coli, Isolation of DNA from plant and animal source, Isolation of plasmids from E.coli pBS and pBS Neo strain, Estimation of DNA and RNA, Restriction Digestion, Transformation, White blue selection, Agarose gel electrophoresis. ** Insulin release assay using pig pancreas, Glucose uptake assay using pig diaphragm. ** Immunological techniques – ELISA, Western blotting. [b][u]COMPUTATIONAL SKILLS [/u][/b]: Microsoft Word, Excel, PowerPoint. [b][u]PERSONAL INFORMATION [/u][/b]: [b][u]Name [/u][/b]: Seema Narasanagi [b][u]D.O.B [/u][/b]: 27 -06- 1990 [b][u]Nationality [/u][/b]: Indian [b][u]Marital status [/u][/b]: Single [b][u]Languages known [/u][/b]: Kannada, English, Hindi, Telugu . [b][u]Interest [/u][/b]: Listening music . I hereby declare that all the information provided by me in this application is correct and factual to the best of my knowledge and belief. [b][u]Place [/u][/b]: Mysore [b][u]Date [/u][/b]: 13/09/13 Yours sincerely, Seema Narasanagi[/QUOTE]
