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[QUOTE=Guest;212380]Neeraj Verma
 biotechindia123 AT gmail.com 
 biotechindia124 AT gmail.com 
 
[b]Note [/b]: Kindly communicate through mail or call after 8.0 P.M 
Objective To build a long-term promising career with a good firm, which encourages professional growth through a wide range of challenging assignments, use my skills and knowledge of biotechnology and allow me to gain expertise & enhance my skills.
 - Therapeutic Proteins and Monoclonal antibody purification.
- Characterization of protein.
Work Experience 4 Years, 2 M
Batch Scale Worked Up to 200 L scale
ork profile Working as Research associate since April 2008 till date with Cadila healthcare group. During last 4 year of my career in the field of research, I got opportunity to work on various therapeutic proteins as well as Monoclonal antibodies derived from E. coli (Bacteria), CHO cells derived proteins, and currently working on purification of Monoclonal antibodies. 
My key role in the project is to separate the desired active molecule from the crude mixture with high degree of purity and recovery. The process of separation primarily involves cell separation by either by centrifugation or filtration or a combination of both the techniques. The critical separation of the biologically active molecule involves various column chromatography techniques with resins having specific property either charge, affinity, hydrophobicity or based on size of the biomolecule.
I am also actively involved in characterization of the purified protein / anti-bodies used for the treatment of cancer, by various biological tools i.e SDS gel, peptide mapping, analysis of charged variants by IEF, purity analysis by HP-SEC, estimation of protein by different method. Some of the biomolecule I worked with are as below –
- Monoclonal Antibodies 
- EPO & PEGEPO
Achievements - Preclinical study completion of Mab projects & DBT document submission 
- Qualified GATE examination in year 2004 (91.65%, AIR 395). 
- Faced facility audit from DCGI
- Technology Transfer 
Skill-set
 Protein Purification -
Column chromatography – Affinity, Ion Exchange, Hydrophobic interaction, Gel filtration.
Column Packing – Series of Millipore and GE column
Virus clearance - Virus filtration of monoclonal antibodies.
Characterization -
Vertical gel electrophoresis, Isoelectric focusing (IEF), Western blotting, Protein estimation, Peptide mapping.
Instrument handling – 
Series of Akta systems (AKTA Pilot, Purifier, Explorer, Basic, Prime), Centrifuge (Batch and continuous), Cell disrupter, Spectrophotometer, TFF system, Gel Doc, Rotavapour, gel units, Autoclave.
Documentation – 
SOP’s , STP’s, BMR & protocol preparation
Writing skill of Electronic Note Book (ELN)
Educational 
Qualification Class / Degree Institute / University Percentage Year Rank
 M. Tech. (Biotechnology) M.I.T.S. Gwalior 79.90 2007 1st
 M. Sc. (Biotechnology) C.S.J.M.U. Kanpur 69.70 2005 1st
 B.Sc. Chemistry, Zoology) C.S.J.M. Kanpur 60.10 2001 1st
 12th UP Board, Allahabad 60.00 1997 1st
 10th UP Board, Allahabad 60.60 1995 1st
Extra-Curricular Activities
 - Attended Good Laboratory Practiceiver “GLP training” at Zydus Reearch Centre, Ahmedabad
- Attended 91st Indian science congress 2004 held at Punjab University, Chandigarh
- CertifCertificate course in “Advanced course on Biotechnology and Intellectual Property” from World Intellectual Property Organization (WIPO), specialized organization of United Nations, Geneva, Switzerland.
- Attended workshop on “Management of intellectual property rights in
Biotechnology” organized by Biotech Consortium India Limited, New
 Delhi
Academic Research Experience Five months dissertation work in area of “Protein purification & denaturation” under the supervision of Dr. Vinod Bhakuni, Molecular and Structural Biology division, Central Drug Research institute Lucknow India
Five months project work in the area of “Molecular Biology” under the supervision of Dr. Bushra Malik, Lecturer, and department of Biotechnology, Madhav Institute Of Technology & Science, Gwalior MP.
Three months project work in the area of “Clinical Immunology” under the supervision of Dr. Nuzhat Husain , Neuropathology, Genetics and Molecular Biology lab, King George Medical University, Lucknow, India
[b]DECLARATION [/b]:
I hereby certify that all the information provided above is true to the best of my knowledge and belief. 
[b]Place [/b]: Ahmedabad 
[b]Date [/b]: Neeraj Verma[/QUOTE]

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