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[QUOTE=hemalathaijt;196456][B]Kakatiya University Practical
Question Bank B.Sc. Biotechnology III year 
Paper – III
Time: 3 hrs Max.Marks:50[/B]

[B]Model Question List [/B]: [URL]http://www.kuwarangal.com/FormsList.aspx?Page=2[/URL]
[B]Model Question Paper[/B] : [URL]http://www.kuwarangal.com/Uploads/OnlineForms/BIOTECH_UG_III_YR_BIOTECH__MODEL_PAPERS_2010-11.pdf[/URL]

Q.I. a) Isolation of DNA from plant cells 8 Marks
b) Isolation of DNA from animal cells
c) Isolation of DNA from bacterial cells

Q.II. a) Analysis of DNA by agarose get electrophoresis 8 Marks 
b) Restriction digestion of DNA

Q.III .a) Preparation of competent cells of bacteria 8 Marks 
b) Bacterial Transformation 
c) Selection of Transformants under pressure (antibiotics)

Q.IV. a) Immuno diffusion Test 8 Marks 
b) ELISA Test
c) Microagglutination using microtiter plates (ABO & Rh blood groups) 
d) Viability Tests of cells/bacteria (Evans blue test or Trypan blue test)
e) Coomb’s Test

Q.V. Spotting (4 x 2 ½) 10 Marks

Q.VI. Record 4 Marks

Q.VII. Viva-voce 4 Marks[/QUOTE]

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