Practical Model Question Paper Biotechnology Kakatiya University : www.kuwarangal.com

[hemalathaijt]

Kakatiya University Practical
Question Bank B.Sc. Biotechnology III year
Paper – III
Time: 3 hrs Max.Marks:50

Model Question List : http://www.kuwarangal.com/FormsList.aspx?Page=2
Model Question Paper : http://www.kuwarangal.com/Uploads/On...RS_2010-11.pdf

Q.I. a) Isolation of DNA from plant cells 8 Marks
b) Isolation of DNA from animal cells
c) Isolation of DNA from bacterial cells

Q.II. a) Analysis of DNA by agarose get electrophoresis 8 Marks
b) Restriction digestion of DNA

Q.III .a) Preparation of competent cells of bacteria 8 Marks
b) Bacterial Transformation
c) Selection of Transformants under pressure (antibiotics)

Q.IV. a) Immuno diffusion Test 8 Marks
b) ELISA Test
c) Microagglutination using microtiter plates (ABO & Rh blood groups)
d) Viability Tests of cells/bacteria (Evans blue test or Trypan blue test)
e) Coomb’s Test

Q.V. Spotting (4 x 2 ½) 10 Marks

Q.VI. Record 4 Marks

Q.VII. Viva-voce 4 Marks