Organisation : Kerala Public Service Commission
Announcement : Syllabus
Designation : Laboratory Technician

Home Page : https://www.keralapsc.gov.in/
Download Syllabus Here : http://www.indianjobtalks.com/upload...atory-tech.pdf

Laboratory Technician Grade II Exam Syllabus :
Unit I :
Veterinary Parasitology :
Introduction to Clinical Parasitology, laboratory- Microscopy– Basic concepts in Veterinary helminthology, entomology, acarology and protozoology. Handling of equipments routinely used in lab like centrifuges, weighing balances, glass and plastic ware etc.

Preparation of stains, like Hematoxylin stains, Acetic acid carmine, ZN carbol fuchsin, fixatives like 10% formalin, Boiun’s fluid, Carnoy’s fluid, Schaudinn’s fixative etc and mounting media like lactophenol, glycerol, Preparation of sporulation solution,etc.

Techniques of collection of clinical materials including faeces, blood and urine-preparation of solutions and scientific handling of clinical samples–Collection and preservation of clinical materials as well as parasite specimens obtained, antemortem and post mortem-acquaintance with specimen maintenance.

Diagnostic morphological features of nematodes, trematodes,cestodes affecting animals, flies, fleas, lice, bugs, ticks and mites affecting animals-life cycle stages of helminths with special emphasis to demonstrable stages of parasitic helminths- Processing of samples, identification of ova of helminths in faeces, urine, nasal discharges etc.

Methods for identification helminth larvae from blood samples by Knott’s technique and histochemical staining. Micrometry- copro culture techniques and copro antigens- Detection of Anthelmintic resistance, Preparation of temporary and permanent mount of helminths. Fecal egg count reduction test and Egg hatch assay.

Role of vectors in disease transmission-collection, processing, identification and preparation of permanent mounts-Xeno diagnosis- examination of skin scrapings-identification of adults and developing stages-processing and preservation of arthropods in dry and wet mounts.

Temporary and permanent staining techniques of tissue protozoa including iodine staining, Heidenhan’s Iron Haematoxylin staining- preparation of blood smear, buffy coat smear, lymph node smear–Quantitative buffy coat system–wet film examination-Diagnostic morphological features of different classes of haemoprotozoa and protozoa like Giardia, Entameoba and Balentidium coli -coccidiosis - cryptosporidiosis - avian malaria-Haemoproteuscolumbae, babesiosis– theileriosis -toxoplasmosis and sarcocystis -Identification of diagnostic stages from secretions and excretions using conventional methods-recent trends in protozoan disease diagnosis from faecal smears- basic serological tests- basic immuno parasitological techniques- Lab animal inoculation for diseases like trypanosomosis, leishmanosis in animals, throat swab examination for trichomonosis in pigeon.

Unit II :
Veterinary Pathology :
General introduction to hematology, Preparation of site and Collection of blood from different species, anticoagulants, separation of serum, TEC, TLC, DLC, ESR, PCV, Hb, Erythrocyte indices, Reticulocytes, Platelet count, Bleeding time and Clotting time, DLC – Staining – Common stains for hematology-Peculiarities of animal blood evaluation Collection, Preservation &despatch of clinical materials like urine, milk and semen.

Physical, Chemical and Microscopical examination of urine, milk and semen. Serum biochemistry-serum enzymes and electrolytes, blood sugar, serum proteins. Evaluation of serum enzymes like AST, ALT, ALP, Serum bilirubin, serum protein, sugar, Sodium, Potassium, Copper, Calcium, Urea etc.

Function tests of liver and kidney Urinalysis- routine chemical tests, microscopical examination of sediments, Milk –various tests for mastitis, cell counts, chloride tests, white side test, California mastitis test, milk culture and antibiotic sensitivity test.

Semen, physical and microscopic examination, Preparation of semen smears and staining. Preservation of postmortem samples and biopsies, fixatives, documentation, cytology.

Histopathology- Techniques in histopathology-Processing of tissues, section cutting, Staining, Preparation of specimens for cytology General Principles of staining, Factors influencing staining –Simple stains, compound stains , Special stain, Classification of stain, Preparation of stains and slides, Staining procedures. Mounting, Labelling and Storage of slides, Inventory management of chemicals, Museum technology / preparation.