Guru Nanak Dev University Bridge Course Syllabus For IInd Year : web.gndu.ac.in

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University : Guru Nanak Dev University
Announcement : Syllabus
Stream : Life Sciences

Home Page : http://web.gndu.ac.in/index.aspx
Download Syllabus Here : http://www.indianjobtalks.com/upload...DGE-COURSE.pdf

Bridge Course in Biotechnology Syllabus :
BTL 501 : Fermentation Technology
UNIT-I
Introduction :
Introduction to Fermentation processes, Types of fermentation processes, batch fermentation processes and its kinetics, plug flow fermentation process and its kinetics, continuous fermentation processes and its kinetics, Fed batch fermentation processes and its kinetics, factors affecting fermentation processes.

UNIT-II
Isolation & Screening :
Isolation, Screening, characterization and preservation of industrially important micro organisms, growth and production media, isolation & screening methods, improvement program of industrial microorganisms, mutants and mutational program for industrial micro organisms, recombinant of industrial micro organisms, preservation and maintenance of industrial micro organisms, quality control of preserved industrial micro organisms.

UNIT-III
Media for Industrial Fermentation :
Introduction, typical media, medium formulation for growth and production, energy source, carbon source, nitrogen source, minerals, growth and production factors. Oxygen requirement,antifoam, medium optimization.

UNIT-IV
Down stream Processing :
Recovery and purification of fermentation products, removal of microbial cells, cell aggregation and flocculation, foam separation, precipitation, filtration, different types of filters and filter aids, industrial centrifugation and industrial centrifuges, basket centrifuge, tubular bowl centrifuge,solid bowl centrifuge, multi chamber centrifuge, disc bowl centrifuge,.

Cell disruption : physical method, chemical method, liquid-liquid extraction, aqueous two phase extraction, super critical fluid extraction chromatography, membrane separation processes, Drying and crystallization.

UNIT-V
Effluent Treatment and Fermentation Economics :
Disposal, treatment processes, physical chemical and biological treatments, aerobic processes. Trickling filters, towers, biological aerated filters (BAFs), rotating drums, fluidized bed system, activated sludge processes, anaerobic treatment, up flow anaerobic sludge blankets (UASB), Byproducts, fermentation economics & market potential.

BTL 502 : Genetic Engineering Applications
UNIT – I
Plant Transformation technology : T he basis of tumour formation, hairy root, features of T1 and R1 plasmids, mechanisms of DNA transfer, role of virulence genes, use of T1 and R1 as vectors, binary vectors, use of 35S and other promoters, genetic markers, use of reporter genes, reporter gene with introns, methods of nuclear transformation, viral vectors and their applications, multiple gene transfers, vectorless or direct DNA transfer, particle bombardment, electroporation, microinjection. Transgene stability and gene silencing. In planta transformation, transgene validation.

UNIT – II
Applications of plant transformation for productivity and performance : Herbicide resistance for phosphoinothricin, glyphosate, sulfonylurea, atrazine; Insect resistance: Bt Genes, non-Bt like protease inhibitors, alpha amylase inhibitor; Virus resistance: coat protein mediated, nucleocapsid gene; Disease resistance; chitinase, 1-3 beta glucanase, RP antifungal proteins, thionines, PR proteins; Nematode resistance, Long shelf life of fruits and flowers: use of ACC synthase, poly-galactouranase, ACC oxidase; Male sterile lines: bar and barnase systems; Carbohydrate composition and storage: ADP glucose pyrophosphatase, terminator gene technology.

UNIT – III
Molecular pharming (farming) : edible vaccines, therapeutic proteins, Nutritional quality: golden rice, protein, vitamins. T-DNA & transposon tagging, promoter trapping, activation tagging. Chloroplast transformation: advantages, vectors, success with tobacco and potato;

UNIT – IV
Molecular marker-aided breeding : RFLP maps, linkage analysis, RAPD, SSLP markers, STS, microsatellites, SCAR (sequence characterized amplified regions), TE anchors, SSCP (single strand conformational polymorphism), AFLP, QTL, map-based molecular marker assisted selection, Application of RFLP in forensic, disease prognosis, genetic councelling, pedigree, varietal analysis, etc., animal trafficking and poaching, germplasm maintenance, taxonomy and biodiversity.

UNIT-V
Transgenic applications of Trehalose, Proline, NAC, CBF, HVAI, HSF, Na-H antiporter

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