www.florenceeducation.com FLORENCE ACADEMY Delhi : BIOTECHNOLOGY FACULTY

[Admin]

FLORENCE POLYTECHNIK/FLORENCE ACADEMY/FLORENCE CONVENT/FLORENCE EDUCATION GROUP
http://www.florenceeducation.com
http://www.florenceinfotech.com

Keywords: NEW DELHI, BIOTECHNOLOGY, BIOINFORMATICS, BSCBT, PGDBT, MSCBT, BSCBI, MSCBI, PHYSICS, CHEMISTRY, BIOLOGY, ZOOLOGY, BOTONY, IT, SKILLS, EDUCATION, FACULTY, TEACHER, TRAINER, LECTURER, PROFESSOR, COUNSELLOR, INSTITUTES, UNIVERSITY, EXPERT, ACADEMIC

Designation: BIOTECHNOLOGY ? BSCBT/PGDBT/MSCBT -FACULTY/TEACHER/LECTURER(FT/ PT/ V)
Experience: 0 - 5 Years
Location: Delhi, Delhi/NCR
Compensation:
SALARY BEST IN MARKET- REQUIREMENT IS URGENT, IF IN DELHI CONTACT PERSONALLY WITH YOUR RESUME/CV/BIODATA AND TWO PP SIZE PHOTOGRAPHS ASAP TO FILL THE FREE JOB APPLICATION FORM. OUTSTATION CANDIDATES TO APPLY WHEN READY TO RELOCATE AT THEIR OWN COST.
Education: UG - Any Graduate - Any Specialization
PG - Any PG Course - Any Specialization
Industry Type: Education/ Teaching/Training
Functional Area: Teaching, Education
Posted Date: 04 Oct 2008

Job Description
TO TEACH STUDENTS OF BIOTECHNOLOGY OF LEADING PTU LEARNING CENTRES. DELIVER LECTURES & CONDUCT ACADEMIC EVALUATION. TO PROVIDE CAREER GUIDANCE & MOTIVATE STUDENTS FOR BEST SKILLS. RESPONSIBLE FOR CURRICULUM PREPARATION & COURSE COVERING ETC.

Desired Candidate Profile
DIPLOMA/ DEGREE/ BACHELORS/ MASTERS/ PG/ DOCTORAL IN BIOTECHNOLOGY OR ALLIED SUBJECT. RELEVANT EXPERIENCE IN TEACHING FIELD, COMMAND OVER THE SUBJECT IS DESIRABLE, ENERGETIC AND MOTIVATED, ABILITY TO WORK IN A FAST-PACED ENVIRONMENT

Company Profile
AN EDUCATIONAL GROUP RUNNING MULTI CAREER INSTITUTES, PRENURSERY TO PRIMARY SCHOOLS, DANCE/MUSIC ACADEMY AT PRIME LOCATIONS AT NEW DELHI. LC OF PUNJAB TECHNICAL UNIVERSITY, DOEACC & REPUTED GOVT. RECOGNIZED UNIVERSITIES. FRANCHISEE OF REPUTED BRANDS.

Executive Name: GAURAV VARSHNEYA/SAURABH VARSHNEYA

Address:
Florence Polytechnik
4, VAISHALI ENCL,IInd FLOOR, PITAMPURA METRO ROAD, NEW DELHI-88. PH.91-11-65378610-13.
D-61, SOUTH EXT.-I, BENGALI SWEETS ROAD, NEW DELHI-49. PH.91-11-65959996-98.
GATE NO.-2, KAPIL VIHAR, PITAMPURA, NR. KOHAT METRO STATION, NEW DELHI-34. PH.91-11-65197711-12.
New Delhi - NEW DELHI ,INDIA 110088

Email Address: florencejobs@rediffmail.com, jobs@florenceeducation.com

Telephone: 91-11-65378610-13,65959996-98,65197711

Fax:
91-11-42455588

Reference ID:
BIOTECHNOLOGY ? BSCBT/PGDBT/MSCBT -FACULTY/TEACHER/LECTURER (FT/ PT/ VISITING)

[sravs0411@gmail.com]

respected
im sravya.pothineni completed M.SC BIOTECHNOLOGY so im intrested in lecturer job if any vacciency in your institute please respond me.im very great ful to your institute

[Guest]

i m kavita aggarwal. i have completed my B.Tech biotechnology and m resident of delhi and looking forward in teaching job. if u have job or me please let me know. my email id is kaveeta.aggarwal AT gmail.com

[tomshe@yahoo.com]

IAM A 24 YEARS OLD FRESH POST GRADUATE IN BIO-TECHNOLOGY LOOKING FOR A SUITABLE OPENING.

[Guest Poster]

RESUME

VIKASH KUMAR
M No.: -+91-9730859402
E-mail: - dev_aryan20 AT rediffmail.com , devaryan20 AT gmail.com


CAREER OBJECTIVE: -

Intend to structure my growth in place with the ever-changing corporate environment. Make my learning curve to move in a linear fashion along with the growth of my technical skills coupled with overall personality development in order to face the challenging items ahead.

EDUCATIONAL QUALIFICATION: -

B.pharma graduate from Karnataka college of Pharmacy under Rajeev Gandhi University of Health & Science, Bangalore by the year 2001-2005.
Intermediate from J.N. College Madhubani under Bihar Intermediate Examination Council, Patna by the year 1999-2001
Matriculation Under Bihar Secondary Examination Board, Patna by 1999

EXPERIENCE: -

Presently working with Matrix Laboratories Ltd. Nashik (A subsidiary of Mylan Inc. USA) the worlds third largest Generics Company, since Feb 2008 as a Quality Assurance Officer (Validation Team).
Previously associated with USV Ltd. Daman As Quality Assurance Officer from Nov 2005 to Feb 2008.


COMPANY PROFILES: -

Matrix Laboratories Ltd (A subsidiary of Mylan Inc. USA), A USFDA, MHRA, MCC, WHO Geneva & India approved Company. The New Mylan (Mylan + Matrix + Generic business of Merck) is the world’s third largest pharmaceutical organization in Generics business with more than 3000 crores turnover. The company having footprints in more than 140 countries and territories.

USV Ltd, Daman A USFDA, NDA Uganda, Nam Finland and various other regulatory agency approved company having more than 800 corers turnover, manufacturing various dosage form like Tablets, capsules and small volume injectables. USV is the first Company to file the Generic version of the Minocycline Tablets.

JOB CAPABILITIES AND EXPOSURE: -

Performing and reporting Qualification and Validation studies. (Preparation and Compilation of Cleaning Validation, Water System Validation protocol and Reports). Participation as validation team member in the Process Validation, Equipments qualification, HAVC system Validation.
Trending of the Water results for the monitoring of the water system
Perform in process checks on shop floor.
Give line clearance
Sampling of intermediates/finished products.
Batch card audit and compliance.
Compiles data for Annual Product Quality Review.
Preparation of Departmental SOPs.
Vendor evaluation and periodic vendor evaluation and maintain the records for the same.
Collection of non-conformance from production floor and assure that corrective and preventive actions are taken and documenting the same.
To ensure the conformance of systems and work practices with the Standard Operating Procedures based on Current Good Manufacturing Practices & Documentation.
Packed goods inspection.
Monitoring the scrap handling and disposal activities.
Monitoring the balance and all IPQA instruments calibration activity throughout the plant.
Issue and control specifications, STPs, SOPs of the plant.
Login any market complaints and maintain records.
Login any planned/unforeseen deviation & change control proposal and maintain records.
Maintain control samples and records.
Issuance and control of BMR, BPR, formats SOP, STP etc.

PERSONAL PROFILE: -

Name : Vikash kumar
Father’s Name : Sri Baidyanath Jha
Date of Birth : 5th July 1984.
Nationality : Indian
Marital Status : Single
Hobbies : Playing Volleyball, cricket, and football & reading books
Motto : Do the work right, first time, every time.
Strength : Hard work, Commitment towards work, Confidence, and
Grace of my elders
Computer Knowledge : M.S.-office, SAP & Internet application.
Other achievements : Various certificates in Volleyball, athletics, skit etc...
Language Known : Hindi, English, Maithili (reg. language)

COMMUNICATION :


Address : C/O:- Nisha N. Lalwani; Miheer Apt.
Flat No. 11; Near Ramnath Kalantri Mangal Karyalaya
Datt Mandir Road; Nashik Road, Nashik

Permanent address : At&po: -Nahar Bhagwatipur
Via: -Rampatti
Dist: - Madhubani (Bihar)
847 236

DECLARATION :

All the information’s provided in this resume is authentic as per my knowledge and belief.

Date: -

Place: -


VIKASH KUMAR

[Guest Poster]

hello sir,
i am pankaj rathore did my pg in biotechnology (field of interest biochemistry and immunology) from amity university,and looking for lecturership job .so if possible do give me the chance to groom your students as well as organisation with my knowledge.
thanking you

[Guest]

i m Shaheen jamal.l. i have completed my M.SC(HOns.) biotechnology and looking forward in teaching job. if u have job or me please let me know. my email id is jamal.shaheen AT yahoo.com .

[Guest]

hi ,i am amrit completed msc biotech degree .i am looking for as lecturer job if u have any vacancy plz contact me on my email amrit.2@rediffmail.com

[Guest]

Hi There,
I am Akhilesh Kumar Gupta, completed M. Tech in Bio Electronics and looking for faculty opening. Please let me know if this position is still persist.
my email id
akhileshddu@gmail.com

[Guest]

I m Neha chauhan completed my post graduation in M.Sc. Biotechnology.......
i m looking for a lecturer job in Delhi-NCR region .if u hv any vacancy pls contact me on my e-mail address-neha.chhn@gmail.com.

[Guest]

Hello
I am Anjali Priyadarshini just finished my Ph.D. I am looking for an opening in academics as Assistant Professor.

[Guest]

I , Arti Kumari, have completed my post graduation in M.Sc. Biotechnology in jan 2011 with distinction.......
i m looking for a lecturer job in Delhi-NCR region .if u hv any vacancy pls contact me on my e-mail address artimishra85@gmail.com .

[Guest]

my self post graduation in biotech , compliting my phd in bioinformatiocs shortly filled a form in your institution for faculty let me know present position.

ph no -- 011-

[Guest]

Nitima Suryavanshi

(M.Sc. Bioinformatics)

E-mail : nitimasuryavanshi22 AT gmail.com

Carrier Objective :
To utilize my analytical and investigative skills for obtaining a position in Biological research, Clinical research and Biotechnology research that offers ample scope for learning and professional growth while being resourceful and innovative.

Work Experience :
Currently working with Blue Net Vista as a web designer since Dec. 2009. Blue Net Vista is a leading web services company in India.

Educational Qualifications :

- Master of Science (M.Sc.) in Bio-Informatics at Dept. of Bioinformatics, Integral University Lucknow in 2009 with 65.96 %

- Bachelor of Science (B.Sc.) with Z.B.C. from D.D.U. Gorakhpur University, Gorakhpur in 2007 with 58.33 %.

- Intermediate (10+2) from U.P. Board with 60 % (PCB) in 2004.

- High School (10th) from U.P. Board in 2002

Areas of Interest :

Sequence analysis, , molecular docking, 3D Modeling, Proteomics & Genomics, Protein function and structure analysis, phylogenetics, protein-protein interaction, computer aided drug discovery and design, system biology, biochemical pathways modeling and simulation, s Functional genomics and proteomics, microarray analysis

Bioinformatics Skills :

- Genomics, Proteomics and Transcriptomics : Basic knowledge of genomics, Proteomics & Genomics theories, fundamentals and theories of Transcriptomics
- Biological Databases : Knowledge of working on NCBI, PDB, and theoretical knowledge of KEGG, Pfam, OMIM SCOP, CATH, GenBank, PROSITE etc. Proteomics tools of SWISS-PROT, EMBL, ClustalW, BLAST and FASTA algorithms.
- Bio Molecular Structure Prediction (Computer Lab) : Good knowledge of softwares like SPDBV, Gold.
- DNA Microarray : Knowledge of DNA Microarray, methods and their application in various fields. Computational Analysis of DNA microarray expression.
- Computer-Aided Drug Design : Theoretical knowledge of Computer Aided Drug Designing. Docking.
- Biophysical Methods : Basic Knowledge of NMR, EMR, Microscopic techniques, TEM, SEM etc.

Technical Skills :

- Operating Systems : WINDOWS-XP,VISTA, LINUX(FEDORA CORE)
- I. T. Skill : ORACLE 9.1 ,My SQL,D.B.MS.,PHP, Java ,C , Python.
- Packages/Software : MS-Office 2003-2007
- Bio-informatics Software : Autodock 4.0 & Modeller
- Some Other : Dreamweaver, HTML,CSS, Adobephotoshop.

Project Undertaken :
Structural Analysis of Gamma-Hexa Chloro Cyclo Hexane Dehydro Chlorinase Anzyme and its rule in Lindane Bio Degredation Pathway” from Indian Institute of Toxicology Research Center. Supervisor : Mr. Nikhil Garg and Mr. Shailendra Gupta, Scientist ‘E-2’Computer Center of the Institute (Email: itrc AT itrcindia.org Tel.-0522-2436144).

About Project :

Lindane is an Organochlorine pesticide. Organochlorine pesticides are man-made insecticide, fungicide and antimicrobial chemicals. Gamma- Hexachlorocyclohexane dehydrochlorinase (Lin A) is a unique dehydrochlorinase enzyme that has no homologous sequence and for which the evolutionary origin is unknown.
This project work is an attempt to generate an improved 3D-Structure of this enzyme to be used in Lindane bioremediation scheme by using MODELLER 9V6 & analyzing the result by using PROCHECK.

Awards/Seminars Attended :

1. Attended the National Workshop & Seminar in Biotech Park, Lucknow on “Principles And Application Of Bio-informatics In biotechnology”
(Sponsored by Department of Biotechnology, Govt. of India)

2. One month summer training on “Bio-informatics tools and Techniques” in BCS-InSilico Biology (A Subsidiary of Biopreen Consultant Services) under the supervision of Mr. Santosh Kumar (HRD Head of BCS-InSilico Biology, Lucknow).

Declaration
I affirm that the information given above is true and correct to the best of my knowledge. I hereby declare that all the information furnished above is true to the best of my knowledge.

Place : New Delhi
Signature
Date :
Nitima Suryavanshi

[Guest]

Nisha Kapoor
Email : nisha AT mcbl.iisc.ernet.in , nisha2208 AT gmail.com ,
EDUCATIONAL QUALIFICATIONS
- Ph.D in Microbiology and Cell biology from Indian Institute of Science, Bangalore, India, August 2005-2010.
- M.Sc. (1st Class) Biotechnology from Guru Nanak Dev University, Amritsar, Punjab, India, July 2003-2005.
- B.Sc. (1st Class) Basic Sciences from Jammu University, India, June 2000-2003.
SCHOLARSHIPS/AWARDS/ACHIEVEMENTS
- Qualified All India M.Sc Biotechnology Research fellowship programme through combined Biotechnology Entrance Test conducted by JNU (2003-2005).
- Qualified Indian Council of Agricultural Research fellowship (ICAR, 2003) for Masters of Science.
- Qualified Joint CSIR-UGC National Eligibility Test (NET) June 2005.
- Qualified National Level Test for Ph.D fellowship at Indian Institute of Science, Bangalore, India, 2005.

RESEARCH EXPERIENCE
- Ph.D in Microbiology and Cell biology department from Indian Institute of Science, Bangalore from August 2005 under the guidance of Dr. K.N Balaji. The main focus of research work is to delineate different signaling pathways involved in M. bovis BCG induced MMP-9 and Novel Granzyme B inhibitor expression.
- Underwent three-month training at IARI, New Delhi, India under supervision of Professor Anupam Varma. Basic techniques related to virus isolation, purification and Agrobacterium mediated gene transfer were learnt.
- Carried out six month project entitled “Estrogen Mediated Protection of Differentiation C6 Glial Cells after Heavy Metal (CoCl2) induced Cytotoxicity” under supervision of Prof. Gurcharan Kaur at Guru Nanak Dev University, Amritsar, Punjab, India. Neuroprotective potential of estrogen against the heavy metals induced toxicity in differentiated C6 Glioma cells was studied both by morphological (phase contrast microscopy, immunoflurorscent staining and Hoechst) and biochemical (LDH, Trypan dye exclusion test) assays.
OVERVIEW OF LABOATORY SKILLS
Animal Cell Culture and Related Techniques
- Culture of cells : Handling various human and mouse cell lines, culture of primary mouse peritoneal macrophages, culture of Bone marrow derived macrophages and
dendritic Cells, MACS sorting of cells , Isolation of primary mouse T cells from spleen and lymphnodes.
- Immunoassays : ELISA assays to cytokine release by cells, Radioactive Thymidine incorporation assay.
- Flow cytometry : Flow cytometric staining for membrane associated and intra cellular molecules.
- Gene silencing : siRNA mediated gene silencing in vitro and in vivo.
- Reporter assays : Luciferase assay, ß-galactosidase assay (ONPG assay).
- Cell cycle analysis : MTT Assay, BrdU Incorporation assay.
- Signaling studies : Phospho and regular western blotting, Immuno precipitation.
- Apoptosis Analysis : Single Color FACS (PI staining), Hoechst Staining, DAPI Staining, LDH assay.
- Microscopy : Immunofluorescene, Confocal, immunocytoflourescence, immunohistochemiistry.
DNA / RNA and Protein techniques
- DNA/RNA : Genomic DNA and plasmid DNA isolation, Agarose gel Electrophoresis, PCR, Molecular cloning, Site-directed mutagenesis, DNA End Labeling, RNA isoalation, RT PCR, Real Time PCR, ChIP.
- Protein : Raising Polyclonal antibody, Expression and Purification of HIS fusion proteins, Native and SDS-PAGE, Electrophoretic Mobility Shift Assay, (EMSA), Western blot analysis (Regular and Phospho Westerns), Size exclusion and Affinity chromatography, Immunoprecipitation, Immunohistochemistry.
- Microbial : Bacterial culture, strain maintenance and other general microbiological techniques.
- Animal Handling : Mice, Rabbits were handled according ethical regulations of IISc.
- Computer skills : Internet and web based bioinformatics tools like clustal W, BLAST, MS office. Usage of tools like clone manager, other PCR tools.
CONFERENCE AND PRESENTATIONS
- Participated and presented a poster in conference on “Gene Expreesion and Signaling in immune system” organized by cold spring harbor laboratory, New York from 21st April- 25th April 2010.
- Participated and presented a paper in 36th Annual Conference of Indian Immunology Society organized by National Institute of Mental Health and Neuro Sciences, Bangalore 16th-19th December 2009.
- Participated in IISc Centenary Conference 10th Sir Dorabji Tata Symposium on Mechanisms of Microbial Pathogenesis 10-12 March, 2009, organised by Sir Dorabji Tata Centre for research in Tropical Diseases, Indian institute of Science, Bangalore, India
- Participated in IISc Centenary Conference held during 13th-16th December 2008 at Indian institute of Science, Bangalore, India
- Participated in Seventh Sir Dorabji Tata Symposium on HIV/AIDS Research held during 10-11 March, 2006 organised by Sir Dorabji Tata Centre for research in Tropical Diseases, Indian institute of Science , Bangalore, India
- Conducted ICMR Workshop for M.D students all across India in December 2006.
PUBLICATIONS
1. Kushagra Bansal#, Nisha Kapoor#, Yeddula Narayana, Germain Puzo, Martine Gilleron, Kithiganahalli Narayanaswamy Balaji. #equal first author. PIM2 Induced COX-2 and MMP-9 Expression in Macrophages Requires PI3K and Notch1 Signaling. PLoS ONE. March 2009, Volume 4, Issue 3, e4911.
2. Yeddula Narayana, Kushagra Bansal, Akhauri Yash Sinha, Nisha Kapoor, Germain Puzo, Martine Gilleron, Kithiganahalli Narayanaswamy Balaji. SOCS3 expression induced by PIM2 requires PKC and PI3K signaling. Molecular Immunology. 2009; 46, 2947–2954
3. Nisha Kapoor,Yeddula Narayana, Shripad A. Patil, and Kithiganahalli N. Balaji. Nitric Oxide Is Involved in Mycobacterium bovis Bacillus Calmette-Gue´rin–Activated Jagged1 and Notch1 Signaling. J. Immunol. 2010; 184, 3117-3126.
4. Rashmi Chaturvedi, Kushagra Bansal, Yeddula Narayana, Nisha Kapoor, Namineni Sukumar, Shambhuprasad, Nagasuma Chandra, Saurabh Mishra, Parthasarathi Ajitkumar, Beenu Joshi, V. M Katoch, Shripad A. Patil, and Kithiganahalli N. Balaji. The Multifunctional PE_PGRS11 Protein from Mycobacterium tuberculosis plays a Role in Regulating Resistance to Oxidative Stress. THE JOURNAL OF BIOLOGICAL CHEMISTRY. 2010; 285, 30389–30403.
5. Priyanka Das, Amit Lahiri, Ayan Lahiri, Minakshi Sen, Namrata Iyer, Nisha Kapoor, Kithiganahalli Narayanaswamy Balaji, Dipshikha Chakravortty. Cationic Amino Acid Transporters and Salmonella Typhimurium ArgT Collectively Regulate Arginine availability towards Intracellular Salmonella Growth. PLoS ONE.December 2010, Volume 5 , Issue 12, e15466.
6. Nisha Kapoor and Kithiganahalli N. Balaji. Mycobacterium bovis BCG triggered MMP-9 and novel granzyme B inhibitor in macrophages imparts resistance to CTL lysis (Manuscript in preparation).
7. Nisha Kapoor and Kithiganahalli N. Balaji. NOD2 receptor mediated novel granzyme B expression requires signaling partnership between PI3 kinase and PKC pathways (Manuscript in preparation)
Research Summary
The research focus of my doctoral studies is to explore different mechanisms employed by M. tuberculosis to survive inside host.
Summary of the work
Mycobacterium tuberculosis is a global health threat, infecting about one third of the human population and resulting in an annual casualty of ~2 million people worldwide. In majority of infected individuals, a certain number of macrophages exist that harbor viable mycobacteria, yet most infected individuals do not develop disease thus raising the question of how these bacilli are contained by the host immune system. Interestingly, studies have suggested that as long as host immunity (mediated by effector macrophages and functional T cells) is effective, pathogenic mycobacteria normally do not exhibit gross adverse effects on the health of the infected host thus suggesting a existence of a fine balance between a potentially dangerous pathogen and the host immune system. The delicacy of this balance is often illustrated by the observation that any deterioration of host immunity results in a potentially life-threatening condition of individuals harboring live pathogenic mycobacteria.
My graduate studies towards the Ph.D degree attempts to delineate the molecular mechanisms essential for the initiation as well as maintenance of the above-mentioned immunological balance. Mycobacteria species including Mycobacterium tuberculosis, M. bovis, etc., have evolved diverse sets of sophisticated survival mechanisms to interfere with the ensuing immune response. Macrophages are vital components of both innate as well as adaptive immunity, and among macrophages, classically activated macrophages play crucial roles in resisting a range of infections by producing key effector molecules like NO and are known to participate in host control of the spread of various intracellular pathogens including Mycobacterium sp. NO is a product of arginine metabolism and produced by different isoforms of NO synthases. Among three isoforms, inducible NO synthase (iNOS) is expressed in a variety of immune cells, including macrophages, in response to a diverse set of proinflammatory stimuli. NO resulting from iNOS enzymatic activity is suggested to integrate fundamental cell fate decisions during immune responses. It is well known that NO or its derivatives like peroxynitrite (ONOO2) modify diverse cellular proteins by processes of nitrosylation, nitrosation, nitration, or oxidation. NO mediated S-nitrosylation of cysteine residues of target proteins including various signaling molecules or the nitration of key tyrosine residues to nitrotyrosine is implicated as one of the novel strategies to modulate a variety of signaling events during initiation or activation of inflammatory responses.
My graduate work demonstrated for the first time that NO, a product of iNOS activity, was responsible for M. bovis bacillus Calmette-Gue´rin (BCG)-triggered activation of Notch1 in macrophages through direct regulation of Jagged1 expression as well as in generation of activated Notch1. Further, iNOS activity was a critical factor in TLR2-mediated Notch1 activation as macrophages derived from iNOS knockout (iNOS-/-) but not from wild-type (WT) mice failed to activate Jagged1 expression as well as Notch1 signaling upon M. bovis BCG infection. The loss of TLR2-mediated Jagged1 expression or Notch1 activation in iNOS-/-macrophages was rescued by treatment with NO donor 3-morpholinosydnonimine (SIN1) or S-nitroso-Nacetylpenicillamine (SNAP). Signaling perturbations strongly implicated the role for cross talk among members of Notch1-PI3K and MAPK cascades in M. bovis BCG-TLR2–mediated activation of Notch1 target genes MMP-9 or Hes1. Chromatin immunoprecipitation (ChIP) experiments demonstrated that M. bovis BCG’s ability to trigger increased binding of CSL/RBP-Jk to MMP-9 promoter was severely compromised in macrophages derived from iNOS-/- mice compared to WT mice. These results were consistent with the observation that NO-triggered Notch1 signaling-mediated CSL/RBP-Jk recruitment had a positive regulatory role in M. bovis BCG-induced MMP-9 transcription. Importantly, my study had demonstrated that this mechanism operates in vivo by immunohistochemical expression analysis of activated Notch1 or its target gene products Hes1 or MMP-9 in brains of WT or iNOS-/- mice that were intracerebrally infected with M. bovis BCG. Further, activation of Notch1 signaling in vivo was confirmed only in granulomatous lesions in brains derived from human patients with tuberculous meningitis as opposed to healthy individuals validating the role of Notch1 signaling in Mycobacterium pathogenesis. Briefly, my graduate work identified NO as the pathological link between TLR2 and Notch1 signaling, which regulates the relative abundance of various immunopathological parameters including MMP-9 in macrophage
Macrophages are principal mediators of initiation as well as activation of host inflammatory responses to tuberculosis infection. Albeit mycobacteria reside within phagolysosomes of the infected macrophages, envelope glycoconjugates like Lipoarabinomannan (LAM), phosphatidyl-myo-inositol mannosides (PIM), Trehalose 6,6'-dimycolate (TDM; cord factor) etc. are released and traffic out of the mycobacterial phagosome into endocytic compartments as well as can gain access to the extracellular environment in the form of exocytosed vesicles. In this perspective, PIM represent a variety of phosphatidyl-myo-inositol mannosides (PIM) 1-6 containing molecules and are integral component of the mycobacterial envelope. A number of biological functions have been credited to PIM2. PIM2 was shown to trigger TLR2 mediated activation of macrophages that resulted in activation of NF-- B, AP-1, and Mitogen-activated protein (MAP) kinases. In addition to pulmonary granuloma-forming activities, PIM2 was shown to recruit NKT cells into granulomas. Further, PIM was suggested to act as adhesins mediating attachment of M. tuberculosis bacilli to non-phagocytic cells. Accordingly, mycobacterial envelope antigen PIM2 could initiate or affect the inflammatory responses similar to mycobacteria bacilli.
As described above, my work demonstrates that TLR2 stimulation of macrophages by M. bovis BCG resulted in the initiation of Notch signaling, which modulated various target genes involved in pro-inflammatory responses. However, possible implications of Notch signaling on immunological parameters associated with interaction of host macrophages with intracellular pathogen like mycobacteria or its novel cell wall antigens remains scanty. Among diverse signaling cascades, Notch signaling pathway is suggested to execute important function during initiation or activation of inflammatory immune responses. In my graduate study, we show that PIM2, a known TLR2 agonist triggered MMP-9 expressions that involved the activation of PI3K and Notch1 signaling. Distinctly, perturbation of Notch1 signaling suggested the involvement of the cross-talk with members of PI3K and MAP kinase pathway. The data implicated PI3K and Notch1 signaling as obligatory early proximal signaling events during PIM2 induced MMP-9 expression in macrophages. These findings, in our opinion will provide new insights into mechanisms by which different signaling pathways that are activated by pathogenic mycobacteria are integrated in a cross talk that modulates a defined set of effector functions in macrophages.

[Guest]

MAYANKA JHOL
mayankajhol87 AT yahoo.co.in
OBJECTIVE :
I am seeking a challenging position in the field of Research and Development in Biotechnology, with an organization/institute that offers good advancement potential, where I can contribute my skills for its success & synchronize with new technology professional growth while being resourceful, innovative and flexible.
PERSONAL SKILLS :
Comprehensive problem solving abilities, excellent verbal and written communication skills, Ability to deal with people diplomatically, willingness to learn, Team facilitator, A very good temperament.
EDUCATIONAL QUALIFICATION :
1. AT M.TECH. LEVEL
Qualification Board \ University Year Of Passing CGPA
1st year MRIU 2009-10 8.0
3rd sem MRIU 2010-11 7.97
4thrd sem MRIU 2011 Result awaited
2. AT B.TECH. LEVEL
Qualification Board \ University Year Of Passing Percentage
B.tech. Raj. Univ. 2009 73%

2. AT SCHOOL LEVEL
Qualification Board \ University Year Of Passing Percentage
Secondary CBSE board 2003 74 %
Senior Secondary CBSE board 2005 59 %

TRAINING\WORKSHOP UNDERTAKEN :
1. 30 days practical training at NATIOINAL INSTITUTE OF PLANT GENOME RESEARCH, JNU, NEW DELHI
- PROJECT UNDERTAKEN : Study of Terpenes and Terpene biosynthases in bioinformatics.
2. 45 days practical training at NATIONAL PHYSICAL LAB., CSIR, NEW DELHI.
- PROJECT UNDERTAKEN : Fabrication and Characterization of Polypyrrole Films for Glucose & Cholesterol Biosensors
3. 6 months project at INSTITUTE OF GENOMICS & INTEGRATIVE BIOLGY, CSIR, NEW DELHI
- PROJECT UNDERTAKEN : Isolation of xylan and lignin degrading bacteria and using them to treat effluent from pulp & paper industry.
4. 2 months industrial training at Hash Biotech Labs Pvt. Ltd.
APPLICATION SOFTWARE AND PROGRAMMING LANGUAGES :
Operating systems Windows 98 , 2000 , XP, Vista.
Bioinformatics tools Rasmol,, pdb, blast ,fasta
CO-CURRICULAR ACTIVITIES :
Poster presentation
Participation : twice (B.E. 3rd sem, 5th sem)
First prize : 2006
Modal presentation []: participated once(7th sem)

[Guest]

Msc qualified in biotechnology.i have 3years of lab experience and looking for biotech jobs.email id- shibbiotech AT gmail.com