Model Question Paper University Of Pune www.unipune.ac.in : MSc BioTechnology

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Document Described : University Of Pune - Model Question Paper
[4038] - 11
M.Sc. - I (Sem. - I)
BIOTECHNOLOGY
BT - 11 : Biological Chemistry (I)
(Old Course) (2005 Pattern)
Time : 3 Hours] [Max. Marks :80

Instructions to the candidates:
1) Question No.1. is compulsory.
2) Attempt any four questions from Q.2 - Q.7.
3) Neat diagrams must be drawn wherever necessary.
4) Figures to the right indicate full marks.

Model Question List : http://www.unipune.ac.in/university_.../paperSci1.htm
Model Question Page : http://www.unipune.ac.in/university_...gy_oct2011.pdf

Q1) Write notes on any four of the following: [20]
a) Biologically important vitamins.
b) Secondary structure of proteins.
c) Coenuples and cofactors.
d) Gene of Eukaryotes and Prokaryotes.
e) Importance of pH in biological system.
f) Fate of glucose in cytosol under aerobic condition.

Q2) a) Explain the reactions leading to ß - oxidation of palmitic acid. [8]
b) Define amino acids. Write in detail classification of amino acids. [7]

Q3) a) Write the principle, applications of SDS - PAGE. [8]
b) Discuss the forces involved in stabilizing the testiary structure of protein. [7]

Q4) a) Discuss in detail citric acid cycle of carbohydrate metabolism. [8]
b) Which radioisotopes are used in biological system? Explain the applications of radioisotopes. [7]

Q5) a) How protein biosynthesis takes place? Explain in detail. [8]
b) Discuss in detail the structure and functions of homopolysaccharides.[7]

Q6) a) Discuss the mechanism of oxidative phosphorylation. [7]
b) How substrate concentration affects the enzyme activity? Explain Michaelis Menten equation. [8]

Q7) a) Discuss in detail Transcription process. [8]
b) What are different chromatography used in protein purification. [7]