BPharmacy University Of Pune Model Question Paper : www.unipune.ac.in

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Document Described : University Of Pune - Model Question Paper
[4055] – 302
Third Year B.Pharmacy Examination, 2011
3.2 : PHARMACEUTICAL BIOTECHNOLOGY (2008 Course)
Time : 3 Hours Max. Marks : 80

Model Question List : http://www.unipune.ac.in/university_...erPharSci1.htm
Model Question Page : http://www.unipune.ac.in/university_...ew_oct2011.pdf

SECTION – I
1. A) Explain basic steps involved in genetic engineering. 5
B) Write scope and application of biotechnology. 5
OR
1. What is genetic engineering ? Draw various steps involved in genetic engineering and elaborate applications of biotechnology to pharmacy. 10

2. Attempt any five : 15
a) What is vector ? Enlist different types of vectors.
b) Write function of restriction enzyme with examples.
c) Elaborate role of alkaline phosphatase.
d) What is role of terminal transferase and DNA ligase ?
e) Elaborate expression vector.
f) What are linkers and adaptors ?
g) Give an account of host system in genetic engineering.

3. Write short notes (any three) : 15
a) DNA hybridization
b) Complementary DNA
c) PCR
d) Gel electrophoresis
e) Gene therapy.

SECTION – II
4. What is plant tissue culture ? What are different types of PTC ? Write applications of PTC. 10
OR
4. Give an account of gene transfer in plants. 10

5. Attempt any five : 15
a) Enlist different types of vaccines.
b) What is DNE vaccine ?
c) Elaborate suspension culture.
d) Write in short different methods of sterilization.
e) Write in short about Animal culture.
f) Write short account of culture media for PTC.
g) Elaborate enzyme immobilization.

6. Write short notes (any three) : 15
a) Radio immunoassay
b) Monoclonal antibodies
c) Transgenic animals
d) Synthesis of HUMAN INSULIN
e) Growth hormone synthesis by rDNA technology.