BTech Industrial Biotechnology www.annauniv.edu Anna University : Model Question Paper

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ANNA UNIVERSITY :: CHENNAI – 600 025
MODEL QUESTION PAPER
VI - SEMESTER
B.TECH. INDUSTRIAL BIO-TECHNOLOGY
IB341 - BIOINFORMATICS

http://www.annauniv.edu/academic/qp4.htm
http://www.annauniv.edu/academic/mqp5sem-ug/ib341.doc

Time: 3hrs Max Marks: 100

Answer ALL Questions
PART – A (10 x 2 = 20 marks)

1.Given 2 strings ”SWALN” and “CRWLNA” give an alignment, edit distance and edit transcript for it. If the cost of replacement is – 1 and the cost of an indel is –2 what is the weighted edit distance?
2.2 alignments have the same edit distance. Will the score of the alignments also be the same?
3.What are sequence motifs? Give an example.
4.Name 2 protein databases. What is cross-referencing in databases.
5.What is basecalling? How do they resolve sequencing errors in genome sequencing projects?
6.What does the number 62 signify in the substitution matrix BLOSUM62?
7.Define raw score, bit score and e-value in BLAST.
8.What are the features of a multiuser operating system?
9.What is annotation?
10.Construct a profile with the following multiple sequence alignment
R G M_ A
R G AG A
R M MG _
M G _G M
A G AR A

PART – B (5 x 16 = 80 marks)
Question 11 is compulsory

11.Describe an algorithm for global alignment of strings. Align the two strings LLNCDR and LRCDN globally using the Needleman and Wunsch algorithm
12.Discuss the ultrametric tree construction. What kind of data is used to build such a tree? Do the data points 6,3,3 qualify to be ultrametric?
(OR)
What are the heuristic methods employed for database searching. Explain Gapped Blast. What are the applications of such database searches.
13.What is the biological basis for multiple sequence alignment? Explain the various applications of multiple sequence alignment.
(OR)
What are the various parameters used in insilico gene finding approaches? Name two software used for gene finding.
14.Write short notes on
a)Shotgun DNA assembly
b)PSI BLAST
(OR)
Write short notes on
a)Gap penalty
b)UPGMA clustering method
15.What are the challenges in bioinformatics in the post genomic era?
(OR)
Discuss physical and genetic mapping procedures.