12th Std / Plus Two / +2 www.cisce.org : Biotechnology ISC Specimen Question Paper

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Document Described : Biotechnology Question Paper, ISC Question Paper

BIOTECHNOLOGY
PAPER 1
(THEORY)
(Three hours)
(Candidates are allowed additional 15 minutes for only reading the paper.
They must NOT start writing during this time.)
Answer Question 1 (compulsory) from Part I and five questions from Part II.
The intended marks for questions or parts of questions are given in brackets [ ].

http://www.cisce.org/isc_XII_SpecimanQP_2012-13.html

Year 2013 Biotechnology ISC Specimen Question Paper link :
http://www.cisce.org/ISC%202013%20Sp...technology.pdf

Year 2012 Biotechnology Paper II ISC Specimen Question Paper link :
http://www.cisce.org/ISC%202012%20Sp...0Paper%202.pdf

PART I
Answer all questions.
Question 1
(a) Mention any one significant difference between each of the following: [5]
(i) Western bottling and southern bottling.
(ii) Pyranoses and furanoses
(iii) RNA polymerase and DNA polymerase.
(iv) Primary and secondary culture.
(v) Codon and anticodon.
(b) Answer the following questions: [5]
(i) What is optical isomerism?
(ii) What are termination codons?
(iii) What is meant by central dogma?
(iv) Who discovered enzyme restriction endonucleases?
(v) Why is Agrobacterium tumifaciens called as the natural genetic engineer of plants?
(c) Write the full form of the following: [5]
(i) MCS
(ii) NBPGR
(iii) AFLP
(iv) SCP
(v) EST
(d) Explain briefly: [5]
(i) Prosthetic group
(ii) Synthetic seeds
(iii) Palindromic sequences
(iv) Restriction sites
(v) Single strand binding proteins

PART II
Answer any five questions.
Question 2
(a) What are carbohydrates? Classify them giving examples. [4]
(b) Explain the steps involved in the post translational modification of proteins. [4]
(c) Enlist four characteristics of a genetic code. [2]
Question 3
(a) Explain the following biochemical techniques: [4]
(i) Electrophoresis
(ii) Centrifugation.
(b) Explain the different methods of freeze preservation of germplasm. [4]
(c) What is meant by the term insertional inactivation? [2]

Question 4
(a) Explain how cell culture technology is useful in crop improvement. [4]
(b) Explain a method used for DNA sequencing using ddNTPs. [4]
(c) Why are cDNA libraries preferred over geneomic libraries? [2]
Question 5
(a) Explain semiconservative mode of DNA replication. [4]
(b) Mention the ways by which the apparatus and instruments used in a tissue culture laboratory can be sterilized. [4]
(c) What are coenzymes? Name any two of them. [2]
Question 6
(a) Mention the chief characteristics of embryonic stem cells. [4]
(b) Explain any four chemical properties of lipids. [4]
(c) Differentiate between: [2]
(i) Blunt ends and sticky ends.
(ii) RAM and ROM

Question 7
(a) What do you understand by gene regulation? [4]
(b) Write the different roles that proteins accomplish in a cell. [4]
(c) What are liposomes? [2]
Question 8
(a) Write short notes on: [4]
(i) Virus mediated gene transfer.
(ii) Microinjection.
(b) Discuss the principle and the procedure of the PCR technique. [4]
(c) What are start and stop codons? [2]
Question 9
(a) Explain the role of computer in new drug development research. [4]
(b) Write a note on different types of sequence analysis programs. [4]
(c) Name any four institutes in India, which deal with biotechnology. [2]

BIOTECHNOLOGY
PAPER 2
(PRACTICAL)
(Three hours)
(Candidates are allowed additional 15 minutes for only reading the paper.
They must NOT start writing during this time.)
Answer all questions.
The intended marks for questions or parts of questions are given in brackets [ ].

Question 1
(a) You are provided with a culture of bacteria isolated from curd. Proceed as follows:
Pick up a bacterial colony with a tooth pick or an inoculation loop and spread it on to a glass slide. Add two drops of crystal violet solution (1%) and gently warm it on a hot plate at 40oC until it becomes dry. Wash the smear with water to remove excess stain. Next, stain the slide with about 2 ml of iodine solution for 1 minute. Wash the smear with acetone. Now, counterstain the smear with saffranin solution (0.5%) for 1 minute. Again wash the smear with water and observe the slide under microscope.
Answer the following questions:
(i) Write your observation when the slide is placed under the microscope. [1]
(ii) Identify the technique used above and mention its use in the laboratory. [1]
(iii) Explain the principle on which this technique is based. [1]
(iv) Name the bacteria present in the curd. [1]
(b) Perform the experiment described below and answer the questions that follow:
Take a ripe mashed banana (about 2N piece) into a beaker and add 30 ml of extraction fluid (E.F.) provided to you. Incubate the beaker at 60oC for 15 minutes. Stir gently with a glass rod. Filter 5 ml of the cooled content into a test tube and add 5 ml of cold 90% alcohol to it. Allow the test tube to stand for 10 minutes.
Answer the following questions:
(i) What do you observe in the test tube after 10 minutes? [1]
(ii) Give two uses of genomic DNA in molecular biology experiments. [1]
(iii) Name a manor contaminant found in the above sample. [1]
(iv) Name two equipments used for quantitative estimation of DNA. [1]

Question 2
A milk sample for the isolation of proteins has been provided to you. Take 25 ml of the sample in a 50 ml flask and proceed as follows:
(i) Warm the sample in a water-bath set at 35oC. Note the pH of the milk sample using a pH meter or a pH paper.
(ii) Next, add 0.4 N HCl drop-wise until the protein starts coagulating. Note the pH at which maximum separation of protein takes place. Filter the solution through a thin muslin cloth to separate out the protein. Show the protein isolated by you to the Visiting Examiner.
Answer the following questions:
(a) (i) Report the initial pH of the milk sample. [1]
(ii) Report the pH at which the protein gets coagulated. [1]
(b) Name the protein and carbohydrate present in the above sample. [1]
(c) Explain the basic principle involved in the above isolation. [2]
(d) Mention two precautions to be taken while conducting the above experiment. [1]

Question 3
Identify the instruments/photographs of the instruments (1 – 4) commonly used in a biotechnology laboratory. For each instrument write:
(a) The names. [2]
(b) One important structural feature. [2]
(c) Specific use. [2]

Question 4
Show the following to the Visiting Examiner for assessment.
(a) Project. [7]
(b) Biotechnology Practical File. [3]