M. Pharm Syllabus : www.mtu.ac.in Mahamaya Technical University

[mariammal]

M. Pharm (Pharmacology)
(First Semester)
PHAR-511 Modern Analytical Techniques

Unit - 1 :
UV-Visible Spectroscopy: Principle of UV-Visible Spectroscopy, Chromophores and their interaction with UV-visible radiation and their utilization in structural, qualitative and quantitative analysis of drug molecules. Woodward-Fieser rule, use of shiff reagents for elucidation of structures. Fundamentals of Optical Rotatory Dispersion. Cotton effect curves, octant rule, circular dichronism.

http://www.mtu.ac.in:8080/web/guest/sylibus
http://www.mtu.ac.in/documents/18/14...ession+2010-11

Unit - 2 :
Infrared Spectroscopy: Infrared radiation and its interaction with organic molecules, vibrational mode of bonds, instrumentation and applications, effect of hydrogen bonding and conjugation on absorption bands, interpretation of IR spectra. FTIR and ATR, X-ray diffraction methods.

Unit - 3 :
Nuclear magnetic resonance spectroscopy: Magnetic properties of nuclei, field and precession, chemical shift concept, isotopic nuclei, reference standards and solvents. H NMR spectra, chemical shifts, multiplicity, coupling constants, integration of signals, interpretation of spectra,
decoupling-double resonance and shift reagent methods. Principles of FT-NMR with reference to 13C NMR, free induction decay, average time domain and frequency domain signals. Spin-spin and spin-lattice relaxation phenomenon. Protein noise decoupled spectra. Nuclear overhauser enhanced 13C NMR spectra, their interpretation and
application. APT and DEPT techniques. Introduction of 2D NMR techniques, COSY, with application.

Unit - 4 :
Mass spectrometry: Basic principles and brief outline of instrumentation. Ion formation, molecular ion, metastable ion, fragmentation process in relation to molecular structure and functional groups. Relative abundance of isotopes, chemical ionization, FAB, ESI, Maldy, GCMS and other recent advances in mass spectrometry.

Unit - 5 :
Chromatographic techniques: Principles of separation and application of Column, Paper, Thin layer and Gas chromatography, HPLC, HPTLC, Size exclusion chromatography, Affinity chromatography, Electrophoresis. Instrumentation of HPLC, Preparative and micropore columns, Reverse phase columns, Mobile phase selection and detectors in HPLC. Instrumentation and application of DCCC. Biological standardization: Bioassay & Radioimmunoassay: ELISA, Radioimmunoassay of drugs
like Digitalis & Insulin

Books Recommended:
1. Willard, H.H., Merrit, L.L., Dean, J.A., Settle P.A., Instrumental Methods
of Analysis, Van Nostrand.
2. Skoog, D.A., Heller, F.J., Nieman, T.A., Principles of Instrumental Analysis, WB Saunders.
3. Hunson, J.W., ed. Pharmaceutical Analysis, Modern Methods, part A & B, Marcel Dekker.
4. Schirmer, R.E., ed. Modern Methods of Pharmaceutical Analysis, Vols 1, 2. Boca Raton F.L., CRC Press.
5. Mann, C.K., et al., Instrumental Analysis Harper & Row.
6. Jaffe, H.H., Orchin M., Theory & Applications of Ultraviolet Spectroscopy, Willy.
7. Silverstein, Spectrometric identification of Organic Compounds, Willy.
8. Bovey, F., Jelinski, L., Miran, P., Nuclear Magnetic Resonance Spectroscopy, Sau: Diego Academic.
9. Stothers, J.B., Carbon-13 NMR.Spectroscopy, Academic.
10. Gordy, W., Theory & Applications of Electron Spin Resonance, Willy.
11. Haswell, S.J., ed. Atomic Absorption Spectroscopy, Elsevier.
12. Ardrey, R.E., Pharmaceutical Mass Spectra, Pharmaceutical Press, London.
13. Budzikiewicz, et al., Interpretation of Mass Spectra of Organic Compounds, Holden- Day San Francisco.
14. Beckett and Stenlake, Practical Pharmaceutical Chemistry, CBS.
15. Stahl, E., Thin Layer Chromatography- A laboratory Handbook, Springer-Verlag
16. Giddings, J.C., Principles and Theory- Dynamics of Chromatography, Marcel Dekker.
17. Sethi, P.D., Quantitative Analysis of Pharmaceutical formulations, CBS Publishers, New Delhi.
18. Kemp William, Organic spectroscopy, Pal grave, New York.
19. Kalsi, P.S., Spectroscopy of organic compounds, New age publishers, New Delhi.
20. Gross - Mass Spectrometry
21. WHO - Quality Assurance of Pharmaceuticals, Vol. I, II.
22. Sethi, P.D., HPLC, Quantitative Analysis of Pharmaceutical Formulations, CBS Publishers, Delhi.
23. Sethi, P.D., HPTLC, Quantitative Analysis of Pharmaceutical Formulations, CBS Publishers, Delhi.
24. Haffmann, Chromatography.
25. Sethi and Charcgankar, Identification of Drugs in Pharmaceutical Formulations by TLC.
26. Robert D. Braun, Introduction to Instrumental Analysis.
27. Wilfried, M.A. Niessen- Liquid Chromatography-Mass Spectrometry.
28. Harry G. Brittain, Spectroscopy of Pharmaceutical Solids.
29. George, S., Steroid Analysis in Pharmaceutical Industry.
30. Higuchi, Pharmaceutical Analysis.
31. Bidingmeyer, Practical HPLC Methodology and Applications.
32. Hoffmann, Mass Spectrometry: Principle and Application.
33. Scott, Techniques and Practice of Chromatography.
34. Wilkins, Identification of Microorganism by Mass Spectrometry.
35. Wu, Handbook for Size Exclusion Chromatography and related Techniques.