www.rdunijbpin.org Rani Durgavati Vishwavidyalaya : Microbiology Syllabus

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RANI DURGAVATI VISHWAVIDYALAYA, JABALPUR
SYLLABUS PRESCRIBED FOR THE DEGREE OF THE BACHELOR OF SCIENCE
(From 2011-12 onwards)
B.SC FIRST SEMESTER MICROBIOLOGY
Paper- General Microbiology (MM Theory 70 + CCE 30)

UNIT I :
Introduction to Microbiology:
History; Spontaneous generation conflicts; Germ theory of disease; Development in medical microbiology; Development in non-medical microbiology- soil and agriculture microbiology, food and industrial microbiology, molecular biology; Scope of microbiology; Applications of
microbiology in human welfare; Development of microbiology in India and abroad: Contributions of Antony van Leeuwenhoek, Alexander Fleming, Edward Jenner, Louis Pasteur, Robert Koch, Salmen Waksman, Joseph Lister, M.S. Swaminathan, G.P. Talwar, T.S. Sadasivan, C.V. Subramaniyam and R. N. Singh in microbiology.

http://www.rdunijbpin.org/syllabus.asp
http://www.rdunijbpin.org/biosyl%5CB...%202011-12.pdf

UNIT II :
Diversity of Microbial World A:
Classification; General characteristics and structure of Bacteria (Eubacteria and Archaebacteria); Morphology of bacteria; Ultrastructure of bacterial cell; Surface appendages- flagella, pilli, prostheceae and stalk; Surface layers of bacteria- sheath, glycocalyx and cell wall; Internal cell structures- cell membrane; Internal membrane system- Mesosomes and Gas vacuoles; Cytoplasmic matrix- Ribosomes, Nucleoid and cytoplasmic inclusions, Dormant structures- Exospores, Cysts and Endospores (sporulation and germination); Cyanobacteria; Actinomycetes; Mycoplasma; Rickettsia and Chlamydia with emphasis on function of each part components.

UNIT III :
Diversity of Microbial World A:
Classification: Brief introduction to classes of fungi; General characteristics: thallus, mycelial modification, nutrition, heterokaryosis, structure with emphasis on function of each part and components of cell; Reproduction: sexual and asexual reproduction; Economic importance of fungi.
Diversity of Microbial World B:
Classification; General characteristics; Morphology and structure: Morphological groups of phages, phage structure, phage nucleic acids; Virus host; General features of virus reproduction: Lytic and lysogenic cycle and their mechanism; DNA and RNA viruses: T4, TMV, Pox virus,
Prions, Virions, Virusoid and Viriods.

UNIT IV :
Microbiological Methods:
Medium optimization: Nutrient, Characteristics of growth medium, Types of media; Preparation of media; Sterilization- definition, Principle, Conditions influencing antimicrobial action; Mode of action of antimicrobial agents; Physical Agents: High temperature- Thermal death time and
Decimal reduction time; Applications of high temperatures for Destruction of Microorganisms- Moist heat, Boiling water Pasteurization, Dry-heat, Incineration; Low Temperatures, Desiccation-Lyophilization, Osmotic Pressure- plasmolysis and plasmoptysis, Radiation-Ultraviolet light, Xrays,
Gamma rays, Cathode rays. Chemical Agents: Characteristics of an ideal antimicrobial chemical agent; Sterilization, Disinfectant, Antiseptic, Sanitizer, Germicide (Microbicide), Bactericide, Bacteriostasis,
Antimicrobial agent; Criteria for selection of chemical agent for practical applications; Major groups of chemical antimicrobial agents and their mode of action.

UNIT V :
Isolation and maintenance of Microorganisms:
Pure, axenic, mixed culture, strain, isolate, clone- Definitions; Pure culture techniques: Dilution, Plating- pour plate method, spread plate method, streak plate method; Enrichment culture and micromanipulator, Maintenance and preservation of pure cultures: subculturing, overlaying,
cultures with mineral oils, lyophilization, sand cultures, storage at low temperature; Major Microbial Culture Collection Centers in India.

List of Practicals (MM: 50) :
1. Precautions to work in microbiology laboratory.
2. Basic media preparation, autoclaving, cleaning and sterilization of glasswares.
3. Media preparation Liquid media – Peptone water, Nutrient broth. Solid media – Nutrient agar (Agar slant, Agar plate) Enriched Medium – Blood agar, Differential medium – Mac Conkey agar, Enrichment Medium – Selenite F broth, Selective medium – EMB
4. Culture characteristics of Microorganisms on different media.
5. Demonstration of selective and differential media.
6. Isolation of bacteria from air, water and soil by serial dilution agar plating method
7. Isolation of fungi from air, water and soil by serial dilution agar plating method
8. Isolation of bacteria by pour plate method.
9. Isolation of bacteria by streak plate method.
10. Isolation of bacteria by spread plate method.

List of recommended books:
1. Microbiology, Authors- Pelczar, Chan and Kreig.
2. General Microbiology, Authors- Stainer, Ingharam, Wheelis and Painter
3. Biology of Microorganisms, Authors- Brock and Madigan.
4. Fundamental Principles of Bacteriology, Author- A.J. Salle.
5. Introduction to Microbiology, Authors- Ingraham and Ingraham.
6. Microbial Physiology, Authors- Moat and Foster.
7. Tools and techniques in microbiology by Nath and Upadhyay
8. Powar C. B. and H. F. Daginawala (2003).General Microbiology Vol.II; Himalaya Publishing House.
9. Dubey R. C. and D. K. Maheshwari (2004). A Text book of microbiology, 1st Edition; S.Chand and Company Ltd.
10. H.C. Dube (2005) A Textbook of Fungi, Vikas Publishing House.