www.thiruvalluvaruniversity.ac.in Thiruvalluvar University : M.Sc. Applied Microbiology Syllabus

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M.Sc. Applied Microbiology : Syllabus (CBCS)
GENERAL MICROBIOLOGY AND MICROBIAL DIVERSITY

Objectives :
To enable the students to understand the basic knowledge in Microbiology and Microbial diversity.

UNIT-I :
Discovery of Microbial world. Controversy over spontaneous generation. Evolutions of Microbiology with its recent developments in Medicine. Role of Microbes in transformation of organic matter and in the causative diseases. Modern trends in microbial taxonomy.

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UNIT-II :
Microscopy - Its principles and applications. Simple - Compound, Dark field, phase contrast, Fluorescent and Electron Microscopes - SEM, TEM. Principles, Operation and maintenance of autoclaves, Ovens, Centrifuges - refrigerated & ultra speed, calorimeters, spectrophotometers, lyophilizers, deep freezer. Staining methods - Gram, Acid Fast, metachromatic, granules, nuclear staining, capsule, silver impregnation, flagella and other special staining methods. Sterilization and disinfection methods and their quality control.

UNIT-III :
Bacterial morphology, structure and characterization - cellular components of bacteria - sporulation and its mechanics - growth and nutrition - Nutritional requirements - Autotrophs, heterotrophs - enrichment culture - growth curve - Kinetics of Growth - Mathematical expression of exponential growth phase; Measurement of growth and growth yields - Batch Culture - Synchronous growth - Techniques of pure culture.

UNIT-IV :
Classification of bacteria and salient features according to Bergey’s manual of determinative Bacteriology. Microbial diversity in different ecosystems (halophiles, mesophiles, thermophiles, acidophiles, alkalophiles, barophiles and other extremophiles). Structure and function of viruses - classification of viruses - replication of viruses - bacteriophages, plant viruses and animal viruses.

UNIT-V :
Classification of fungi according to Alexopoulus and Mims - cell structure, specialized somatic structure. Reproduction in fungi - Asexual, sexual and parasexual cycle - Life cycles of fungi. Structure and Classification of Algae - ultrastructure and life histories of microalgae belonging to various algal classes. Cyanobacteria, Prochlorales and Cyanelles.

Reference Books :
1. Dubey RC and Maheswari DK (2005). A text book of Microbiology, Revised Multicolour edition, S.Chand Publishers, New Delhi.
2. Purohit SS (2005). Microbiology - Fundamentals and Applications. Student Edition Publishers, Jodhpur.
3. Pelczar & Kreig (2006). Microbiology5th edition. Tata McGraw Hill, New Delhi
4. Powar & daginawala (2005). General Microbiology Vol.I & II 8th Edition, Himalaya Publishing House, Mumbai.
5. Salle, AJ (2001). Fundamentals & Principles of Bacteriology. 7th edition. Tata McGraw-Hill, Davis,
6. Delbecco, Eisen & Ginsburg (1990) Microbiology 5th Edition Harper & raw, New York
7. Gerhardt, Murray, Wood and Kreig 1994. Methods for General and Molecular Bacteriology, ASM Press, Washington.
8. Alexopoulus CJ and C W. Mims.(1993).Introductory Mycology (3rd edition) WileyEastern Ltd, New Delhi.
9. Elizabeth Moore-Landecker. (1996). Fundamentals of the fungi.(4th edition). PrenticeHall International, Inc, London.
10. Conrat HF, Kimball PC and Levy JA. (1988). Virology. II edition. Prentice Hall, Englewood Cliff, New Jersey.
11. Roger Hull (2002). Mathews' Plant Virology. (4thEdition).Academic press-A Harcourt Science and technology company, New York.