www.svuniversity.in Sri Venkateswara University : Biotechnology Model Question Paper

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MODEL QUESTION PAPER
Part- II BIOTECHNOLOGY
Paper – III Molecular Biology, Genetic Engineering and Immunology

Section – I :
Answer ALL questions :
4 x 15 = 60
1. Describe the organization and importance of mitochondrial DNA
OR
Explain the upstream regulatory elements of genes
2. Write an account on post transcriptional modifications of m-RNA
OR
Explain the lac – operon
3. Write about the construction of cDNA libraries
OR
Explain the principle and applications of PCR

http://www.svuniversity.in/news/syll...technology.pdf

4. Describe various reactions involved in antigen-antibody complex formation
OR
Explain various types of hypersensitive reaction.

Section – II :
Answer and EIGHT of the following :
8 x 5 = 40
5. Cot curve 11. Kinases
6. Simple gene families 12. Haptens
7. Wobble hypothesis 13. Autoimmune diseases
8. Peptidyl transferase 14. Cells of immune system
9. RFLP 15. Palindromic sequences
10. Shuttle vectors 16. Antibody diversity
Paper IV Applications of Biotechnology
Unit - 1 Cell and tissue culture technology
1.1 Principles of animal cell culture – culture media, importance of serum – cell lines
propogation and maintenance.
1.2 Stem cell – types, isolation and identification techiniques and applications of stem
cell technology
1.3 Principles of plant cell and tissue culture – composition of MS medium – its
significance – role of plant growth regulators in differentiation – Meristem culture,
clonal propogation of plants – Somatic embryogenesis and organogenesis.
1.4 Clonal propogation of economically importance plants (Banana)
1.5 Production of secondary metabolities through plant tissue culture.
Unit II Production and processing of rDNA products and therapeutics
2.1 Microbial production of rDNA proteins – Insulin – EGF, Sterptokinase
2.2 Large scale production –structure of typical bioreactors – fermentation process –
Optimization of production of rDNA proteins – Control of foam and pH
2.3 Downstream processing – continuous centrifugation techiniques, cell distruption,
purification of rDNA proteins using affinity tags
2.4 Molecular farming – Production of therapeutics in milk – Production of edible
vaccines – Production of Human Growth hormone.
Unit III Environmental Biotechnology
3.1 Introduction to environmental biotechnology; Energy – non-renewable energy
resources – pollution, depletion.
3.2 Non conventional energy sources: Biofuels- Biobutanol, Bioethanol – cellulolytic
production of ethanol – production and significance – Biological hydrogen production
– rodobacter capsulaters and its significance,
3.3 Fermentative production of beverages and organic acids (citric acid)
3.4 Biological nitrogen fixation – mechanism and significance, Biofertilizers and
biopesticides
3.5 Microbial mineralization of xenobiotcs and environmental pollutants –
Biodegradation of petroleum hydrocarbons – application of transgenic microbes –
Super Bug in environmental clean up
UNIT IV Embryo Transfer and Transgenic Technology
4.1 Isolation and preservation of gametes/ embryos – Artificial insemination, in vitro
fertilization, super ovulation – Synchronization of estrus cycle
4.2 Transgenic animal – Production fast growing fish, disease resistant cow, Harward
mouse
4.3 Transgenic plants – Bt cotton, Golden rice
4.4 Gene therapy – principles and applications
4.5 Bio-thics – intellectual property rights – patency

Practical :
1. Over expression of cloned gene
2. Preparation of media, and initiation of callus from any one selected plant species
3. Micropropogation of pants (any one)
4. Anther culture for production of haploid plants
5. Preparation of synthetic seeds
6. Fermentative production of ethanol using bakers yeast
7. Fermentative production of organic acids (citric acids)
8. Isolation and fusion of protoplast
9. Production of hydrogen or biogas using cow/cattle dung
10. Preparation of media and culture of animal cells/tissues
11. Cell disaggregation and cell counting
12. Cytotoxicity of the cells using the dye MTT method
13. Estimation of BOD in water samples
14. Production of alcohol by fermentation and Estimation of alcohol by colorimetry
15. Production of biofertilizers (Azolla)
16. Growth curves of bacteria, Measurement of growth in liquid cultures
17. Quality testing of milk by MBRT

MODEL QUESTION PAPER
Part- II BIOTECHNOLOGY
Paper – IV Applications of Biotechnology
Section – I :
Answer ALL questions :
4 x 15 = 60
1. Describe the principle and applications of in-vitro fertilization and embryo transfer
technology
OR
Explain various methods of gene transfer to produce transgenic animals
2. Write an account on applications of r-DNA technology in agriculture
OR
Explain various sterilization methods used in culture media preparation
3. Describe various good manufacturing practices
OR
Explain the primary and secondary metabolic products of microorganisms
4. Write an account on microbial ore leaching
OR
Describe the use of microorganisms in degrading pesticides and toxic chemicals
Section – II
Answer and EIGHT of the following
8 x 5 = 40
5. Preservation of cell lines 11. IPR
6. Ex vivo gene therapy 12. Production of interferons
7. Molecular pharming 13. Growth hormone
8. Somatic embryogenesis 14. Bioremediation
9. Meristem culture 15. Bioethanol
10. Microprojectile bombardment 16. nif genes