www.nrcpb.org Jobs 2011 New Delhi : Research Associate

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Name of the Organization : National Research Center on Plant Biotechnology (NRCPB)

Company Address: :
National Research Center on Plant Biotechnology
Indian Agricultural Research Institute
New Delhi – 110 012, INDIA
Phone : 011-25848783, 011-25841787, 011-25843554

Name of the Post: : Research Associate

NPTC Project: Resistance to Aphids in Mustard: code 2049/3022,
PI:- Dr. Rekha Kansal

Age Limit : Max. 40 years.

Pay scale : Emoluments: Rs 24,000/- + HRA per month in case of Ph.D completed and Rs 18000/- + HRA per month in case of M.Sc. + 2-3 years research experience.

Qualification and Experience : MSc. with 60% marks in Molecular Biology/ Biotechnology / Biochemistry/ Genetics with 2-3 yrs research experience in Molecular Biology Techniques or Ph.D in any of the above discipline. Research experience evidence in terms of publications must be mentioned.

How to Apply :
Send filled application form and copies of certificate to
Interview will be held on January 4, 2011 at 10:00 a.m. at National
Research Centre on Plant Biotechnology, IARI, Pusa Campus, New Delhi-110012.

Detailed Advertisement : http://www.nrcpb.org/For_NRCPB_websi...sal%5B1%5D.pdf

Application form Download : http://www.nrcpb.org/For_NRCPB_websi...sal%5B1%5D.pdf

Company Profile :
The National Research Centre on Plant Biotechnology was established in 1985 to undertake research, teaching and training personnel in the modern areas of Molecular Biology and Biotechnology. Since its inception, the Centre has grown and has acquired high degree of scientific competence and established excellent research facilities. The Centre is working towards achieving the national priorities of increased agricultural productivity and sustainability.

[Guest-IJT]

Name : Ravindra
Email : ravinaidu2 AT gmail.com
Designation / Skillset : Sr.Research Associte
Dear Sir/Madam,
I, V.Ravidra, by occupation Sr. Research Associate in R&D department in M/s. Premas Biotech ltd, Gurgaon. I have 4.0 years working experience in process development of Bio- therapeutic proteins, Monoclonal Antibody and vaccine purification in Biotech firms.I have completed my Post Graduation in Biotechnology .I desire that my profile is adequate and consider for your requirement. My CV is enclosed and in brief profile has given for your consideration. Hence, request you to give me an opportunity to work in your esteemed organization. I assure you that I will perform my job with hard work and dedication.
Thanking you,
V.Ravindra
Sr. Research Associate
R&D
M/s. Premas Biotech ltd
Gurgaon

Resume :
Dear Sir/Madam,
I, V.Ravidra, by occupation Sr. Research Associate in R&D department in M/s. Premas Biotech ltd, Gurgaon. I have 4.0 years working experience in process development of Bio- therapeutic proteins, Monoclonal Antibody and vaccine purification in Biotech firms.I have completed my Post Graduation in Biotechnology .I desire that my profile is adequate and consider for your requirement. My CV is enclosed and in brief profile has given for your consideration. Hence, request you to give me an opportunity to work in your esteemed organization. I assure you that I will perform my job with hard work and dedication.
Thanking you,
V.Ravindra
Sr. Research Associate
R&D
M/s. Premas Biotech ltd
Gurgaon
CURRICULUM VITAE

OBJECTIVE :
Quest to work in a professional and competent atmosphere involving my experience, skills blended with good amount of enthusiasm and zeal aided with my efforts, to contribute for the growth of the Organization.
EDUCATIONAL CREDENTIALS :
- Post Graduation
M. Sc., Biotechnology (2005-2007). Bangalore University, Karnataka.
- Graduation
B. Sc., Microbiology, Medical lab technology and Chemistry (2002-2005). S.V. University, Andhra Pradesh.
CAREER SUMMARY :
Having around 4 years of industrial experiences in Process development of Bio-Therapeutic proteins and Vaccine purification ,Currently I’m working in “Premas Biotech ltd” Gurgaon. as an “Sr. Research Associate”in R&D.
Comprehensive knowledge and strong background in the area of E.Coli & pichia- pastoris, Mammalian & Hibridoma cell culture purification.
INDUSTRY EXPERIENCE
I have been working with Premas Biotech Limited since Oct- 2010 to present date as a full time as an Sr. Research Associate in R&D.
I have worked with Bharat Biotech International Limited since AUG 2007 to Sep 2010 as a full time Executive in R&D
Job Responsibilities
- Downstream processing and optimization.
- Protein characteristics and purification by various chromatographic techniques.
- Purification of proteins by FPLC Duo Flow (Biorad), Akta purifier & Akta pilot purification system
- Monoclonal Antibody purification.
- Optimization of fermentation parameters.
- Responsible for developing research at expert level through production via operation of pilot plant equipment and process experimentation.
- Performed protein purification, large scale fermentation, and other processes under GMP regulations.
- Refolding of of protein expressed in IB(inclusion body).
- Performed related protein analysis
- Optimizing yields of reaction to maximize product output.
- Shake flask experiments related to productivity improvement.
- Design, plan and perform scientific experiments such as excipient compatibility, formulation and process optimization, and scale-up.
- Designing and developing and implementing robustic process for large scale vaccine and therapeutics purification and formulations.
- Analytical Method Development and Characterization of recombinant therapeutic proteins expressed in E.coli, & pichia pastoris.
- Analytical method validations for the Vaccine and Drug Products. optimizing STPs like Assay, SDS-PAGE, Western Blotting and Immuno detection, and ELISA, for the Stability studies of the Final Bulk and Finished Drug products such as Injectables,
- Provide efficient and robust processes / methods for the manufacture drug products
Work according to appropriate SOP s, GMPs, and safety guidelines
Techniques known :
- Downstream Process :
Centrifugation, Dynomill for cell breaking
Chromatography Techniques (IEX, HIC, IMAC, SEC).
TFF
Ultra filtrations
Protein Chemistry :
Isolation, Characterization of different proteins.
Protein purification techniques - Chromatography and Electrophoresis techniques (SDS- PAGE, Native-PAGE).
Instruments Handled :
FPLC Duo Flow (Biorad)
Akta prime(GE helthcare )
Akta pilot (GE helthcare )
Gel Documentation
PCR
Refrigerated Superspeed Centrifuge (Sorval Dupont, Sanyo & Sigma)
UV-Vis spectropotometry (Elico and Shimadzu)
Electrophoretic set up for Protein and DNA/ RNA (Genei, Amersham, BioRad and BIotek)
Sonicator (Vibra Cell)
DECLARATION :
This is to inform you that the above furnished details are best of my knowledge, and in case if anything is found spurious regarding me then I am liable to be rejected.
(RAVINDRA.V)
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More Information about this submission and submitter :-
___________________________________________________
Submission ID : 4158368
Date & Time : 21st May 2011 1:02 PM (UTC)
IP Address : 123.236.48.132
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Predicted Country : India

[Guest]

Curriculum vitae

Priyanka Das
E-mail :prink.bot AT gmail.com
Education
DEGREE YEAR BOARD/UNIVERSITY MARKS
Ph.D. (Botany) 2006-07
Registered Utkal University Thesis Will be submitted in the month of October, 2011
M.Phil. (Botany)
(biochemistry specialization) 2007 Utkal University 73.5%
M.Sc.(Botany)
( molecular biology and biotechnology specialization) 2005 Utkal University 69.5%
B.Sc.(H) Botany
Chemistry, Seed technology 2003 Utkal University 70.3%
Teaching experience
Two years of teaching experience as lecturer in Botany, at Jupiter +2 Science College, Bhubaneswar.
Research Experience
Did M.Phil studies on Invitro tissue culture and biochemical characterization work, at Department of Botany, Utkal University, Bhubaneswar, India, under supervision of Prof. Manoranjan Kar and Dr. Santilata Sahoo.
M.Phil. topic : In Vitro studies on development and biochemical characterization of Cuscuta reflexa.
Project fellow- 3 years of research experience as project fellow/assistant at institute of Life Sciences, Bhubaneswar
Graduate Studies (continuing) on Transgenic Research & Development of stress tolerant plant by genetic engineering, at Utkal University (Botany department), Govt of Orissa and at Institute of Life Sciences (Department of Biotechnology, Government of India), Bhubaneswar, 751023, India.
One year of research experience in School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Ph.D. Topic : “Characterization of Rice Catalase-B and Catalase-B gene Mutant Products in Transgenic Arabidopsis”.
Guide : Dr. Santilata Sahoo,Utkal University, India, Co Guide: Prof. S.C. Sabat, Institute of Life Sciences, India
Duration : May 2007 to till date (Ph.D. thesis to be submitted in the month of October, 2011), Full time. (Synopsis attached as appendix)
Research Skills
- Biochemistry and Molecular Biology
Spectrophotometery
Microscopy
Electrophoresis
Chromatography
DNA, RNA and Protein isolation from prokaryotes and eukaryotes and their analysis
Southern blot analysis
Northern blot analysis
Gene expression and analysis
Microarray data validation
SSH (Substractive hybridization)
C-DNA library preparation
Polymerase Chain Reaction
Quantitative real Time PCR
Gene sequencing (Both manual and automated)
Cloning
Bacterial transformation and expression
Plant transformation and expression
Protein Purification
Western blot analysis
Microbiological techniques
Tissue culture
Chlorophyll fluorescence measurement and Photosynthetic parameters study
Isolation and characterization of pigment and enzymes from living cells or tissue
- Bioinformatics : Computational analysis of large-scale data analysis, Database mining for DNA and protein sequences, CLUSTALW, phylogeny analysis. MIPS for genome functional annotation, TIGR assembler for gene assembly and finding of contigs and singletons, Transcription factor bindings (TFSEARCH). Use of databanks like NCBI.
- Computer skills : Windows, Linux operating systems, graphics applications (Adobe Photoshop, Corel Draw and PowerPoint), Microsoft office, DNA and protein analysis software, Internet.
- Others : Digital Photography, Autoradiography using Phosphorimager (Fuji). Laser confocal (Leica) microscope, Ultra Centrifuge.
- Laboratory Management : Responsible for ordering and budgeting for laboratory supplies and equipment maintenance in a lab of 10-15 persons. Train laboratory personnel including technicians, undergraduate students. Experience in drafting project proposals, project reports.
Publications
Original Research Papers (Published or to be published in peer Reviewed Journals)
1. Sushmita Sahu, Priyanka Das, Mamata Ray, S. C. Sabat* Osmolyte modulated enhanced rice leaf catalase activity under salt-stress, Advances in Bioscience and Biotechnology, 2010, 1.
ABSTRACT
Change in catalase activity was examined in leaves of rice plant exposed to salinity. Depending on the method of preparation of crude protein extract from leaf and the constituents of the assay medium, a sig-nificant difference in enzyme activity was recorded. Inclusion of sorbitol or mannitol or sucrose in the extraction and enzyme assay medium enhanced the enzyme activity in salt-stressed samples by nearly 1.5-1.8 fold, compared to the activity found in un- stressed plants, which otherwise showed a 50% de-clined activity in leaf extract prepared in buffer solu-tion and assayed in a medium depleted of these sug-ars. In view of the accumulation of osmolytes under saline condition, these observations suggest that the catalase activity is modulated by the osmolytes and maintains a high rate of hydrogen peroxide scaveng-ing property in vivo and serves as the major antioxi-dant enzyme to scavenge the salt-induced formation of peroxide. Therefore, the salt-stress induced ap-pearance of low activity of the enzyme under normal buffer extraction and assay conditions, as reported in literature may represent an apparent than for its real in vivo activity.
2. Priyanka Das - Manoranjan Kar . Santilata Sahoo* In vitro hormone regulated growth and floral induction of Cuscuta reflexa -a parasitic angiosperm, Acta physiologia Plantarum,2010
DOI 10.1007/s11738-010-0613-8
ABSTRACT
The role of different growth regulators in callus induction, shoot regeneration, - oral induction and chlorophyll content of the obligatory parasitic plant Cuscuta re- exa has been studied. Callus development was excellent from the nodal part of the shoot explants in modi- ed Murashige and Skoog (MMS) media supplemented with 2 mg L -1 benzyl adenine (MMS1c). Supplementation of 2 mg L -1 naphthalene acetic acid (NAA) along with MMS1c (MMS2c) was responsible for estimable shoot induction and development in callus. 2,4-Dichloro acetic acid (2,4-D) played a crucial role in the - oral induction of C. re- exa in vitro. MMS supplemented with 2 mg L -1 NAA and 2 mg L -1 2,4-D (MMS3b) supported - oral induction after shooting in vitro. MMS supplemented with 3 mg L -1 2,4-D (MMS4a) rapidly induced - ower directly from the stem explants without showing any elongation of shoot. MMS1c along with MMS3b (MMS5a) showed callus proliferation followed by shoot elongation and - oral induction. In vitro MMS5a grown plants show a sharp increase in the chlorophyll contents. Cytokinin treatment further increases the chlorophyll level of the plant
3. Priyanka Das - Naveen Chandra Joshi* Minor modifications in obtainable Arabidopsis floral dip method enhances transformation efficiency and production of homozygous transgenic lines harboring a single copy of transgene. Advances in Bioscience and Biotechnology –Published (Journal :-Advances in Bioscience and Biotechnology, 2011, April, doi:10.4236/abb.2011.22010).
ABSTRACT
Many researchers have developed various methods for In-planta or floral dip transformation of Arabidopsis thaliana. It is the most simplistic protocol and widely used to produce transgenic Arabidopsis. Here we have developed a little modified transformation protocol to get increased number of transformants with less labor and time consumption. Four types of inoculums (inoculum1, inoculum2, inoculum3 and inoculum4) were used to check the transformation efficiency. Inoculum3 showed the highest rate of transformation among the four types. Percentage of surfactant (silwet L-77) is considered as the crucial factor for the transformation efficiency. 0.07% Twin-20 also acts in same manner as silwet L-77 to increase the rate of transformation efficiency. Glucose instead of sucrose can be used in inoculum to transform Arabidopsis. Keeping the Agrobacterium infected plants for 7-8 hrs horizontally in low light at 280 temperature condition, after vacuum infiltration considered best to get increased number of transformed seeds. Modified protocol produced ~12-14% increase in transformants. Selection pots (kanamycin supplemented soil filled pots) in place of selection plates (Kanamycin supplemented Murashige and skoog agar plates) proved beneficial as no MS medium and no aseptic condition is required for selection of transformed plants. This increase in transformation efficiency consequently increased the percentage of homozygous and single copied stable transgenic lines.
4. Mamata Ray, Panchananda Mishra, Priyanka Das, Surendra Ch. Sabat* purification and expression of Rice catA in E. Coli cytoplasmic fration. Manuscript status – Manuscript resubmitted after 1st revision (Journal of Biotechnology).
Abstract
The advent of recombinant protein technology has allowed producing proteins for medical, pharmaceutical and also for industrial uses including proteins for functional and structural studies. Among the heterologous expression systems used in recombinant protein expression, the prokaryotic host Escherichia coli still remains as the most preferred and routinely used organism. However, the organism often shows incompatibility for expression of foreign proteins in functional form, particularly those with high molecular mass requiring polymeric association for their function. Plant catalase is one such protein, whose recombinant expression in Escherichia coli in functional form has been reported to be unsuccessful till date. In this report, we describe a novel protocol for expression of a heme coordinated functionally active rice plant catalase-A protein in Escherichia coli. The protein was produced using thioredoxin as fusion partner with co-expression of molecular chaperone ‘tig’- the Trigger Factor, only when the cells were cultured at low temperature for a longer time. Functional catalase-A was obtained with exogenous supplementation of d-aminolevulinic acid in the medium. The yield of purified active recombinant protein was nearly 1-2 mg per liter of culture. These results attest to the usefulness of the present expression protocol for improving recombinant plant catalase protein production in Escherichia coli
5. Priyanka Das1- Prosenjit Mondal1- Mamata Ray1. Santilata Sahoo2. Baishnab Tripathy3. Manoranjan Kar2 and Surendra Chandra Sabat1* Rice Catalase-B Protein Over Expression in Arabidopsis Enhances Dry Matter Accumulation and Imparts Resistance to Light and Temperature Stress. Planta Journal. (Under review)
ABSTRACT
Catalase is the principal hydrogen peroxide scavenging enzyme in the cell. Intrinsically the enzyme, however, suffers from photo-inactivation of its activity through direct absorption of light by its porphyrin moiety or through yet an unidentified chloroplast-routed redox signaling. Two Arabidopsis transgenic lines, over expressing a normal and mutated rice catalase-B gene product, where the mutated gene product could function by avoiding light and temperature impairments, were developed. These transgenic lines not only showed high level of enzyme activity, but were also endowed with high biomass and seed yield, when compared to wild type plants. The non-chloroplastic over expression of catalase induces high rate of photosynthesis, with mutant types having maximum light utilization efficiency under high irradiance and both transgenics can function by avoiding photoinhibition of photosynthesis. Importantly, a high level of leaf glutathione reductase activity with elevated level of chloroplast glutathione reductase transcript was evident in transgenic lines suggesting that over expression of an antioxidant enzyme which is non-chloroplastic in nature can protect chloroplast function from light and temperature mediated injuries, possibly through removing the rate-limiting step in glutathione reductase activity of ascorbate-glutathione reductase cycle leading for effective scavenging of hydrogen peroxide in chloroplast.
6. Priyanka Das - Manoranjan Kar - Santilata Sahoo* Modulation of antioxidant activity and pigment content by light and kinetin treatment in Cuscuta reflexa : a parasitic angiosperm. Manuscript prepared (to be communicated).
ABSTRACT
The activities of catalase and peroxidase and the concentration of chlorophyll and carotenoid pigment concentration have been studied in detached Cuscuta reflexa twigs after different duration of light (100µE) and different concentration of kinetin treatment both independently and together and compared with the control (water treated) twigs. Catalase and peroxidase activity were increased the highest (about three fold) than the control twigs, when treated with light and 25µM of kinetin after 24 hrs of time. But only 1-1.2 fold increase in Catalase and peroxidase activity was observed when light (100 µE) or kinetin (25µM) treatment was given independently. Independent light treatment resulted, only a marginal increase in chlorophyll and carotenoid content in cuscuta reflexa twigs. But Light treatment along with kinetin showed a sharp increase (about 2.5 fold) in the chlorophyll and carotenoid content. Again tip portion (~2 cm) of the twig shows increase in catalase and peroxidase activity and chlorophyll and carotenoid content maximum than the lower parts.
9. Transgenic seeds
Transgenic seeds will be submitted to seed bank.
10. Microarray data
Data will be submitted to data bank.
Presentations and conference atteneded
- Presented oral on the topic “Increase in dry matter accumulation and enhanced tolerance to light and temperature stress in transgenic Arabidopsis thaliana over expressing rice catalase-B and its mutant gene product ’’ in international conference on frontiers in biological Sciences,NIT,Rourkela,October 01-03,2010
- Presented oral on the topic “Altered photosynthetic activity in Arabidopsis thaliana over expressing rice catalase-B protein” in national seminar on morden biology and human welfare,march-28-29,Department of Zoology, Berhampur University
- Presented poster On the topic “Anti-oxidant activity of Cscuta reflexa-a parasitic angiosperm” in Orissa Botanical Society held at P.N. College, Khurda during February 25-26,2007
- Presented poster on the topic “In-vitro hormone regulated Floral development of Cuscuta reflexa” in National seminar on emerging trends in plant sciences : morphology and biotechnology, held during 29th and 30th October,2006 in the department of Botany, Ravenshaw college, Cuttack
- Participated in International conference on ETBT-2009 & 6th annual convention of BRSI held at Banaras Hindu University, Varanasi during December 4-6,2009
- Participated in the 2nd annual conference of the cytometry of India organized by the Institute of Life Sciences, Bhubaneswar during Feb 1-15,2009
- Participated in the continuing medical education lecture series as part of the 35th annual meeting of the Indian immunology society organized by the Institute of Life Sciences, Bhubaneswar during Dec 1-14,2008 Institute of Life Sciences, Bhubaneswar during Dec 1-14,2008
- Participated and in the 35th annual meeting of the Indian immunology society organized by the Institute of Life Sciences, Bhubaneswar during Dec 1-14,2008 Institute of Life Sciences, Bhubaneswar during Dec 1-14,2008
Summary of graduate project
Characterization of Rice Catalase-B and Catalase-B gene Mutant Products in Transgenic Arabidopsis
A variant rice catalase recombinant protein has been developed in Dr. Sabat’s group at Institute of Life Sciences; Bhubaneswar by site-specific mutagenesis shows insensitivity towards light and also can function under elevated and also under low temperature regimes (Mondal et al 2008). This gene also has high substrate affinity and high turnover rate as well. Therefore, construction of transgenic crop plant with over expression of such elegant catalase may prove beneficial in adverse condition from viewpoint of plant production.
PROJECT PROPOSAL
The affinity of catalase for H2O2 is very low. In spite of high homologies between catalase sequences and a highly conserved structural organization, among various organisms, the kinetic properties show a striking range of variation. In a comparison of purified catalase from sixteen different organisms (mammalian, fungal and bacteria enzymes) with representatives of all three clades of mono-functional catalases, the apparent Km values were found to vary between 38 and 600 mM (Switala and Loewen 2002). In plants, its’ apparent Km values ranges between 10 to 140 mM (Scandalios 1994; Heinze and Gerhardt 2002). However, the apparent Km for catalase does not correspond to the standard Michaelis–Menten parameter, because the catalatic reaction comprises two partial reactions that depend on the concentration of H2O2, both the formation and the reduction of compound-I. The catalatic reaction does not follow Michaelis–Menten kinetic but the activity increases linearly with H2O2 concentration without saturation. Therefore, existing H2O2 concentration controls catalase activity. Apparent saturation may be caused by an inhibition at high H2O2 concentrations (Beaumont et al. 1990; Lardinois et al.1996; Nicholls et al. 2001). The recombinant rice catalase developed by Dr. Sabat’s group has a Km of - 4 ± 1.2 mM compared to its native form having - 11 ± 2 mM. Thus the variant catalase with such striking attribute (high substrate affinity, and light insensitiveness) may be iconic candidate for gene transfer in plants to make it a stress tolerant to ensure the net productivity

Objectives :
1. Raise transgenic Arabodopsis (up to T3 generation) over- expressing rice Catalase-B and its Mutant.
2. Transgene characterization (Gus analysis, Transgene Integration, copy number and expression analysis).
3. Study of tissue specific transcript level expression of rice catalase gene in transgenic Arabidopsis.
4. Morphology analysis of both transgenic and non-transgenic (WT) plants.
5. Biochemical and physiological analysis of developed transgenics.
- Catalase activity study (Both spectrophotometric and in-gel assay).
- Level of H2O2 in both wild type and transgenic plants.
- Status of other antioxidant enzymes like ascorbate peroxidase, guaiacol peroxidase and glutathione reductase.
- Status of H2O2 producing enzymes like superoxide dismutase, glycolate oxidase and NADPH oxidase.
- Study of photosynthetic parameters of wild and transgenic plants i.e ETR, photochemical and non-photochemical quenching, effective quantum yield, number of open and close reaction centers.
6. Responsiveness of transgenics towards light and temperature stress.
7. Characterization of transgenic plants under salinity stress.
8. Microarray of normal and transgenic plants.
9. Validation of generated microarray data by quantitative real time PCR.
Outcomes of work done :
Superior vegetative growth and tolerance against high light and temperature impairment of transgenics : superiority of mutant CAT-B over normal CAT-B
The results presented in this study clearly indicate that over expression of rice CAT protein through transgenic approach provides an opportunity to develop plants endowed with the capacity to avoid photoinhibitory injury of photosynthetic activity, and also for higher biological yield. Previously, other researchers have also reported similar phenomenology of high biomass accumulation by CAT over expression where CAT was specifically targeted to chloroplast. However, unlike previous reports, we have specifically shown here that non-chloroplastic over expression of CAT enzyme also results in enhanced vegetative growth and significantly high dry mass accumulation, and provides protection to chloroplast machinery against photoinactivation, both at high and low temperatures. The presence of relatively higher number of functionally open PSII reaction centers in transgenics further suggest that both the excitation energy transfer and the optimal operation of Calvin cycle as the terminal electron sink of thylakoid electron flow are highly effective in transgenics under conditions, impacting photoinhibitory effect to WT plants.
Protection capacity of transgenics against photoinactivation of photosynthesis
Although both CAT and PSII photochemistry are photoinactivated under similar conditions of light and temperature regimes in WT plant, they are perhaps mutually exclusive and are independent events. But CAT over expression could protect the photoinactivation of photosynthesis indirectly through institution of high rate of chloroplastic ascorbate-glutathione cycle mediated H2O2 scavenging activity. Identification of the underlying signaling mechanism for such indirect stimulation of chloroplastic antioxidant machinery by high catalatic activity of peroxisomal catalase requires further investigation (see Fukamatsu et al. 2003; Verslues et al. 2007). As documented in this report, the presence of effective utilization of light in photosynthesis at high photon flux density, improved growth, and higher dry matter production in Arabidopsis over expressing mutated rice CatB as compared to normal CatB gene provides an opportunity to transfer the strategy to other plant species for improving growth and productivity accompanied with resistance against light and temperature impairments.
Salinity tolerance of transgenics
The resultant transgenic Arabidopsis plants constitutively expressing CAT-B and Mutant CAT-B were able to grow in the presence of 300 mM NaCl, and were able to form - ower and produce seeds in the presence of 300 mM NaCl. The seeds produced from salt treated transgenics were viable i.e. they were able to grow normally as well as in salinity aonditions. Catalase activity in 300 mM salt treated transgenic Arabidopsis plants was 1.5- to 2.5-fold higher than non-transgenic Arabidopsis plants. Our results clearly indicate that simple genetic modi- cation of Arabidopsis to express rice-derived catalase-B can ef- ciently increase its tolerance against salt stresses. Mutant rice catalase showed better tolerance than normal catalase-B.
Microarray data analysis (Not a part of thesis)
Number of genes related too different pathways of cell metabolism and number of transcription factors are unregulated in both types of transgenic plants which will be submitted in data bank.
May be catalase over expression has a key role in alteration of transgenic metabolism, as a consequence so many genes and transcription factors are up regulated for better function of plant from metabolic view point.
The above details are true for the best of my knowledge

[Guest]

Mridusmita Baruah
mrids18 AT gmail.com
mbaruah AT ignou.ac.in
OBJECTIVE
To seek a creative job that suits my educational qualification and literary temperament, working in a team environment, thereby continuously growing & contributing to the objectives of organization.
EDUCATIONAL QUALIFICATIONS
Ph.D. pursuing with IGNOU
B.Ed. : Gauhati University in the year 2003.
MA : English Literature from Cotton College under Gauhati University in the year 1998, IInd Class with UGC norms.
BA : English Literature from Cotton College under Gauhati University in the year 1995, IInd Class.
12th : From Handique College under AHSEC Board in the year 1992, 1st. Div.
10th : From D.A.V High School in the year 1990 under SEBA Board, 1st. Div.
PERSONALITY FACTOR
High degree of mental maturity, tolerance to stress & able to deliver in a high pressure environment.
Comfort in dealing with people at various organizational levels, friendly disposition and interpersonal skills.
COMPUTER PROFICIENCY
IT literate, good working knowledge of Excel, Power Point, Word & Internet.
PROFESSIONAL EXPERIENCE
Lecturer : Department of English in K.C. Das Commerce College, Guwahati
Job Responsibilities : Apart from teaching the subject English from 11th to Degree students, conducted debates, quizzes, seminars, group discussions & other extra-curricular activities.
Duration of Service : June’1999 to June’2000
PG Teacher : Omkarananda Saraswati Nilayam (ICSE Board), Rishikesh
Job Responsibilities : Taught students from IX Std. to XII Std., conducted Quizzes, Debates & other extra-curricular activities.
Customer Care Officer in Convergys India Services, Pune
Duration :- 15th Mar’2004 to 28th Feb’2005.
Job Responsibilities :-Redressing Customer queries for our respective clients. Won several appreciations from customers for the quality work. Member of the Resolution Desk Team as Floor Support for the other agents.
Customer Care Specialist with IBM, Gurgaon
Duration : 15th June, 2005 to 2nd Sep, 2007.
Project Details : Email based customer support for Sprint-Nextel, the mobile service provider in US.
Assistant Regional Director in Indira Gandhi National Open University, Regional Centre Delhi-1
Job Responsibilities : Successfully handled Admission, Academic Counsellors and project section.
Duration : 1st Jan’2008 to 5th Dec’2008.
Current Occupation : Research and Teaching Assistant (RTA) in Indira Gandhi National Open University (IGNOU), New Delhi.
Profile Detail : (i) Pursuing Ph.D. on Bodo Movement in Assam (Insurgency)
(ii) Face to Face classes to MA students
(iii) Unit Writing, Proof Reading and Editing of Self Learning Materials (SLM) of the University
(iv) Organizing Seminars and Conferences
(v) Teleconferencing sessions to remote learners
(vi) Invigilation duty for the MA examinations
Details of the Paper Presented in Seminars/Conferences :
1. Fifth International Conference on Australia and India : Negotiating Change Jan18-21, 2010 Goa University, Goa “Empowerment of Aboriginal Women in the contemporary Australian Society”
2. National Conference on ‘Techno-Lingual Communication (TLC-2010) on March 27, 2010 Sunderdeep Engineering College, Ghaziabad, UP. “Business Communication courses in the MBA Curriculum : Bridging the Communicative Skills Lacunae”
3. National Seminar on ‘Traditions of Folk in Literature’ from August 30-31, 2010, IGNOU, New Delhi. “Socio-Religious beliefs of the Bodo tribes of Assam”
4. National Seminar on Caste, Democratic Politics & Nation Building : Historical & Contemporary India from 10th -12th March, 2011, IGNOU, New Delhi. “Bodo Society and Casteism : A Retrospect of the Influence of Gurudev Kalicharan Brahma”
5. National Seminar on Teacher Education through Open Distance Learning in the Context of Right to Education form 22nd -24th March, 2011, , IGNOU, New Delhi. “Environmental Education through Open and Distance Learning”
Other Orientation/Workshops/Seminars/Lectures :
6. Attended the Orientation Programme from 8th to 12th December, 2008 at IGNOU, Head Quarters, New Delhi.
7. Attended a workshop on Research Methodology held at Academic Staff College, Gauhati University, Guwahati from August 24th to August 28th 2009.
8. Attended a training Programme on SPSS held at Arya Vidyapeeth College, Guwahati from September 7th to September 11th 2009.
9. Actively participated on the North East India Seminar –Cum-Consultation on "Strengthening Development Processes in North East India : Identifying Scope and Challenges in Development and Governance" held at Scope Convention Centre, New Delhi on 30th October, 2009.
10. Participated in the International Conference on Conflict Management, Peace Economics and Peace Science organized by IGNOU, Gandhi Smriti and Darshan Samiti, New Delhi and United Nations Educational, Scientific and Cultural Organisation (UNESCO) in cooperation with Economists for Peace and Security, Peace Science Society (International) Binghamton University, State University of New York, USA held from 11-13 January, 2010 in New Delhi.
11. Attended a Two Day National Seminar on “Social Research on North East India : Issues and Challenges” organised by North East India Studies Programme (NEISP) Jawaharlal Nehru University, New Delhi on 25th -26th February, 2010 held at JNU, New Delhi.
12. Attended International Symposium on “Celebrating Inter-Cultural Dialogue between North East India and South East Asia” jointly organized by Indira Gandhi National Centre for the Arts (IGNCA), New Delhi and North East India Studies Programme (NEISP) Jawaharlal Nehru University, New Delhi from 17th March, 2010 to 20th March, 2010.
13. Participated in a National Seminar on “Translation & Literature” at Lalit Kala Academy, New Delhi from 20-21st March, 2010.
14. Did anchoring for the IGNOU Silver Jubilee Seminar on “Recent Trends in Astronomy and Astrophysics” by Prof. Siraj Hasan, Director Indian Institute of Astrophysics (Bangalore) held at New Delhi on 22nd March, 2010.
15. Participated in a One Day National Seminar on “Assam and the role of Muslims in the Independent Movement” on 25th March, 2010 at Jamia Milia Islamia, New Delhi.
16. Attended lecture on “Political Economy in Carribean Islands” by former PM Trinidad and Tobago at IGNOU and JNU, New Delhi on 29th and 30th March, 2010
17. Attended the Course Work Workshop on Research Methodologies and Interdisciplinary Perspectives by School of Interdisciplinary and Transdisciplinary Studies, IGNOU, New Delhi from 12th to 23rd April, 2010
18. Participated in the “RTA Meet” organized by North East Centre for Research and development (NECRD), Guwahati from 19th to 21st May, 2010 at Guwahati.
19. Delivered a presentation on “Understanding Conflict” at North East Centre for Research and Development (NECRD), Guwahati on 4th June, 2010.
20. Actively Participated in the National Convention on “Revitalisation, Convergence and Implementation of Nai Talim” at Gandhi Smriti and Darshan Samiti (GSDS), Rajghat, New Delhi on 28th August, 2010 organised by National Council Of Rural Institutes (Ministry of HRD, Govt. of India) Hyderabad.
21. Attended the lecture on “The Challenge of Inclusive development in India: A Labourist Perspective” by Prof.K.P.Kannan organised by Glad AT ignou at New Delhi.
22. Participated in the Training cum Workshop on Research Methods for RTA’s of IGNOU held from September 22-24, 2010 organised by Inter University Consortium for Technology-Enabled Flexible Education and Development (IUC-TEFED), IGNOU, New Delhi.
23. Participated in the Annual Conference on Market Economy and Gandhian Ethics hosted by Indira Gandhi National Open University in collaboration Resource Development, Government of India, Hyderabad held from 29-31 October, 2010 in New Delhi.
24. Attended a lecture on “Terrorism, Political Violence and Human Security: Reflection, Introspection and Prospection” delivered by London based senior BBC journalist Mr. Deepak Tripathi on 23rd February, 2011 at IGNOU, New Delhi.
25. Participated on a two day National Seminar on “Caste and the Census” from 23rd Feb to 24th Feb, 2011 at IGNOU New Delhi.
26. Participated in a Lecture on “Indigenous Peoples and the concept of Sustainability for Economic Development” by Prof. Martin Sanchez Jancowski, University of California Berkeley, USA held on 7th March, 2011 at IGNOU, New Delhi.
27. Attended a lecture on “Sources of Data Collection” by Prof. V. Saravanan on 26th April, 2011 at IGNOU, New Delhi.
28. Participated in the Jawaharlal Nehru Memorial Foundation Lecture at Teen Murti Bhawan, New Delhi on 28th April, 2011. Prof. Irfan Habib delivered his key note address on “Jawaharlal Nehru’s Vision of History”
29. Attended a Training Workshop on “Data Analysis and Report Writing” from 9th to 11th June, 2011 at North East Centre for Research and development (NECRD), Guwahati, organized by NECRD in collaboration with Indian Association for Social Sciences and Health (IASSH), Mumbai.
30. Attended the Prof. G. Ram Reddy Lecture that was delivered by Minister of Road Transport and Highways Dr.C.P.Joshi on “Rural Empowerment and Panchayati Raj Institutions” on 2nd July, 2011 at IGNOU, New Delhi.
31. Attended the World Education Summit 2011 held at The Ashok, New Delhi from 13-15 July, 2011.
32. Attended the eWorld 2011 Forum held at The Ashok, New Delhi from 1-3 August, 2011.
33. Attended the lecture on “Indo-Japanese Bilateral Ties in 21st Century” by Prof. Rajram Panda Senior Fellow IDSA on 29th August, 2011 held at IGNOU, New Delhi.
34. Attended the lecture on “Turbulence in the Arab World” by Ambassador R. Rajagopalan on 21st September, 2011 held at IGNOU, New Delhi.
35. Attended the lecture on “Recent Developments in Afghanistan” by Dr. Gulshan Sachdeva of JNU on 26th September, 2011 held at IGNOU, New Delhi.
36. Attended the lecture on “Migration from North-East to Urban Centres: A Study of Delhi Region” by Dr.Babu P. Remesh on 30th September, 2011 held at IGNOU, New Delhi.
37. Attended the lecture on “One Community Two Nations: Indian Muslims in 1940s” by Prof. Rizwan Qaiser of Jamia Milia Islamia on 18th October, 2011 at IGNOU, New Delhi.
ACHIEVEMENTS
Attended all the 'Grow More' programs (Soft Skills Workshop and Office Manners Workshop held at IBM) to ensure progressive learning.
Have taken a lot of training sessions related to the process and utilized the trainings I have attended.
Conducted writing skills training for the agents across the floor, which was a big success for the process.
Conducted training sessions on Personality Development.
Participated and presented Research Papers in several National and International level Seminars and Conferences.
EXTRA CURRICULAR ACTIVITIES
NCC “C” Certificate Holder in Senior Division Air Wing;
Attended three National Camps:
Hyderabad: Vayu Sainik Camp’92
New Delhi: Republic Day Parade Camp’94
Andaman & Nicobar Island, Port Blair: National Integration Camp’95
INTERESTS & ACTIVITIES
Globetrotting, Helping the needy, Music, Watching movies, Reading, Playing Badminton and Cooking.
OTHER ACHIEVEMENTS
Attended hundred hours training for Allianz Bajaj Life Insurance Company conducted by IRDA (Insurance Regulatory & Development Authority).
All India Best Cadet in National Integration camp’95 at Port Blair
All India Best Singer & Flyer in Vayu Sainik Camp’92 at Hyderabad.
Did Parasailing, Horse-riding, Rafting, Trekking etc.
Did Yoga, Meditation & Pranayama at Rishikesh under Swami Rudradev