Usha Fabs Pvt Ltd Delhi, Gurgaon : Plant Biotechnological/Tissue Culture Laboratory Technician

[mskavi]

Usha Fabs Pvt Ltd Delhi, Gurgaon : Laboratory Technician (Plant Biotechnological/Tissue Culture)

Designation: Laboratory Technician (Plant Biotechnological/Tissue Culture)
Experience: 4 - 5 Years
Location: Delhi, Gurgaon

Job Description:
• Candidate should be B.Sc/M.Sc (Agriculture)
• Preference will be given to the candidate works with the similar field

You can also forward your Cv's to tapan_chakraborty@rediffmail.com

Desired Candidate Profile:
• Knowledge of Computer

Company Profile:
Hotel/Readymade Garments Exporter/Medicinal Plants
Fax No : 0124-2347536, Bijwasan,Delhi

Email Address: tapan_chakraborty@rediffmail.com

Keywords: bsc, agriculture, export, gurgaon,

[nangi ramulu]

CURRICULUM VITAE
N.RAMULU
Email : nangi_dav AT yahoo.co.in
Mobile : 9912071898
9030929436

OBJECTIVE :
To utilize my skills and knowledge to build those works, towards the growth of the organization and meets the company’s objectives and service.

SUMMARY :
Working as Senior Microbiologist in M/S SVEN GENETECH LIMITED, having over seven years of experience in the field of R&D, Process Development and Fermentations.

EDUCATIONAL QUALIFICATIONS :
1 Batchelor of science (B.Sc.) Industrial Micro Biology, Botany,Chemistry from Osmania University in the year 2002.
2 Diploma in medical lab technician(DMLT) in 2000
3 Intermediate (Bi. P.C) from Board of Intermediate Education.
4 S.S.C. (General) from Andhra Pradesh Secondary School Certificate.
5 Diploma in computer application(DCA)

WORK EXPERIENCE :
A) Presently working as sr. microbiologist at SVEN GENETECH LTD (a group company of Jupiter bioscience LTD (R&D ) Hyd from Aug 2003 to till date
B) Worked as a microbiologist at Amyloenzymes Pvt. Ltd (production) Since July 2002 to July 2003
c) Worked As lab technician in kamala hospital (as part time)

AREAS OF EXPERTISE :
MICROBIOLOGY
R&D
PROCESS DEVELOPMENT
FERMENTATIONS
ANALYSIS
QC

PROJECTS HANDELED IN R&D :
Co-q-10 or UBIQUINONE – a Neutraceutical
Taxol -Anticancer drug
Teicoplanine -Antibiotic
Bleomycin Antibiotic
Protease used for bio separation of amino acids
Lipase -Used for bio catalysis
Acylase used for bio separation of aminoacids
Pectinase enzyme
Lipo Protin lipase enzyme
Bio separation of amino acidMethionine, phynylalanine, DL-Arginine etc.

TECHNICAL SKILLS :
Summary of experience :proficient with following techniques
1 Screening of different microorganisms for the production of enzymes,antibiotics, drugs).
2 Production of Biological active compounds(antibiotics, enzymes) from microbs by Submerged, Solid State & Surface solid state fermentation methods
3 Biotransformation of chemical compounds.
4 Research on Production of anticancer drugs by microbs.
5 Production of enzymes(Fungal & Bacterial proteases,esterases,lipases) from microbial source.
6 Extraction of above compounds by Liquid-liquid solvent phase extraction, solvent precipitations.
7 Purification of biological active products by Ion exchange chromatography (Cation & Anion Exchange resin), Silica gel Chromatography.
8 Novel Peptide isolation from microbs and their detection, purification by Preparative HPLC.
9 Detection & Estimation of above compounds by Thin layer (Silica and Cellulose Plates),Paper Chromatography & Analytical Procedures (HPLC, UV Spectrometry)
10 Biological Activity of Purified compounds by Disc Diffusion Technique against Bacterial Pathogens.
11 Antibiotic Assays by Turbidometric & Diffusion methods.
12 Estimation of Enzymes, protein activity by biochemical methods.
13 Isolation of Microorganisms (Fungi, Actinomycetes) from different sources.
14 Maintainance of cultures by different preservation techniques (Subculturing, Lyophilisation, Glycerol stock etc.)

PROCESS :
In the above projects starting from slant inoculation till partial purification of product handled.
1 Isolation of different microorganisms from different samples of water, soil, (Fungi, Bacteria, Actinomycities etc.)
2 fermentation by submerged/solid state fermentation,
3 fermentation can be shake flask level to fermentor level,
4 Analysis of the organism by gram staining, acid fast staining, antibiotic sensitivity test.
Analysis of microbial contamination by plating technique, dilution method, calorimetric method.

Proficient with Biochemical lab techniques :
1 HCG
2 complete blood picture
3 Erythrocyte sedimentation rate
4 Total WBC&RBC count
5 Packed cell volume
6 Hemoglobin percentage
7 Blood sugar
8 Blood urea
9 Complete urine examination
10 Urine sugar
11 Urine creatine

PERSONAL PROFILE :
Name : N. Ramulu
Father’s Name : Shivaiah
Date of Birth : 12th Jan, 1980
Religion : Hindu
Nationality : Indian
Languages known : Telugu, English & Hindu

[Guest]

CURRICULUM VITAE
Mr. MAHESH KUMAR VERMA
e-mail : verma.ma82 AT gmail.com
Career objectives :
Seeking an intellectual and challenging position in esteemed organization to utilize my education, skills and experience.
Education
· M.Sc Agril Biotechnology (2011) Marathwada Agril University MS India (80%)
· B.Sc Agriculture (2009) Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad (U.P.) (75.0%)
· H.S.C. (1999), U.P. Board (50.0%)
· S.S.C. (1997), U.P. Board (65.95%)
Masters Thesis :
“Cloning and characterization of sunflower necrosis virus (SNV) infecting sunflower”.\
Awards
1. Qualified JNU-Combined Entrance Examination-2009 for M.Sc. Agricultural Biotechnology program.
2. Fellowship, Department of Biotechnology, Govt. of India during M.Sc. Agricultural Biotechnology program
Resume of Research work performed :
1. Five Sunflower necrosis virus (SNV) isolates were collected and partially purified from different regions of Marathwada viz. Latur, Parbhani, Beed and Osmanabad districts.
2. Primers specific to coat protein gene (CP), Replicase protein gene (RP) and partial RNA3 fragment. Were designed by using software Primer 3.
3. Isolation of coat protein gene (CP), Replicase protein gene (RP) and partial RNA3 fragment were done by using PCR amplification.
4. Cloning and sequencing of SNV genes -
a. Coat protein (CP) gene, Replicase protein gene (RP) and partial RNA3 fragment
gene of SNV were cloned in E.coli through heat shock method.
b. Chitinase genes from Sunflower, Soybean and Pigeon pea were isolated, cloned into pGEMT vector and sequenced.
5. SCAR marker was developed for detection of species Xanthomonas axonopodis pv. punaceae causing oily spot disease in Pomegranate.
6. SCAR marker was developed for assessment of purity of four hybrids in sunflower.
7. RNA isolation protocol was standardised in Sunflower.
Techniques acquanted :
1. Isolation of genomic DNA from organisms like plant, fungi bacteria and viruses.
2. Isolation of RNA from virus infected leaves of Sunflower and Pigeon pea.
3. Different type of PCR viz. touch down, touch up, asymmetric PCR, colony PCR, Multiplex PCR, Reverse transcription PCR (RT-PCR) and Box PCR.
4. PET vector construct and expression of coat protein gene in E.coli.
5. Horizontal and vertical gel electrophoresis.
6. Molecular Cloning and Genetic Transformation
7. Plant transformation
Bioinformatics experience :
The Clustal X and Clustal W programmes were used for Nucleotide sequence alignment, BLASTn, BLASTp and Primer 3 for designing of primers.
List of Publications :
A. Genes cloned, sequenced and deposited in GenBank at NCBI (www.ncbi. nlm.nih.gov)
--------------------------------------------------------------------------------------------------------------------
Sr. No. Description of genes GenBank Accession
---------------------------------------------------------------------------------------------------------------------
1. Coat protein gene of sunflower necrosis virus (SNV1), Latur1 isolate (JF340380).
2. Coat protein gene of sunflower necrosis virus (SNV2), Parbhani isolate (JF340381).
3. Coat protein gene of sunflower necrosis virus (SNV3), Beed isolate (JF340382).
4. Coat protein gene of sunflower necrosis virus (SNV4), Osmanabad isolate (JF340383).
5. Coat protein gene of sunflower necrosis virus (SNV5), Latur2 isolate (JF340384).
B. Manuscript in process :
1.Cloning and characterization of sunflower necrosis virus infecting sunflower isolated from Marathwada regions of India.
2.An efficient protocol for isolation of RNA from virus infected leave samples of sunflower.
Computer literacy :
“Dynamic Web Design course during M.Sc Agril Biotechnology programme”

[garimakaushik76@gmail.com]

GARIMA KAUSHIK
E-Mail : garimakaushik76 AT gmail.com
CAREER OBJECTIVE
Seeking an Entry level position to utilize my skills in your Organisation.
EDUCATIONAL QUALIFICATION
NAMEOF
EXAMINATION PASSED YEAR OF PASSING BOARD/
UNIVERSITY PERCENTAGE OBTAINED REMARKS
10th
2004
CBSE
70%
Pass
12th
2006
CBSE
66.4%
Pass
Bsc life science
2009
DELHI UNIVERSITY 57.8%
Pass
Msc biotechnology
2011
C.C.S UNIVERSITY
70%
Result
Awaited
PROJECTS UNDERTAKEN
Worked as a trainee in “Jawaharlal Nehru University under Professor & Dean Neera Bhalla Sarin” in school of life sciences, in a plant and tissue culture lab on “Overexpression of GlyII for the Development of salt stress Vigna mungo via Agrobacterium mediated transformation”.
KEY SKILLS
- DNA Isolation
- Polymerase chain reaction
- RNA Isolation
- Reverse transcriptase PCR
- Plasmid Isolation
- Protein isolation
- Enzyme assay
- Agrobacterium mediated transformation in plants
- Electrophoresis
- Various Tissue Culture Techniques
ATTRIBUTES
- Won first prize in Rangoli Competition on topic-“ACID RAIN” in B.Sc. (IIIrd year) Delhi university.
- Won first prize in Rangoli Competition on topic-“SAVE EARTH” in B.Sc. (IIIrd year) Delhi university.
- Won third prize in Cartoon making on topic-“FUTURE OF BIOTECHNOLOGY” in B.Sc. (IIIrd year) Delhi university.
- Attended National Symposium on “Molecular Cell Biology” 24 – 25 November, 2009 organized by SHIVAJI COLLEGE (University of Delhi), sponsored by DBT.
- Stood FIRST in poster presentation in national symposium on “MODERN APPROACHES AND INNOVATIONS IN BIOTECHNOLOGY”14-15 NOVEMBER 2010 Organized by college of applied education &health sciences ,Meerut.
COMPUTER APPLICATIONS

Operating Systems[]: Windows 98/2007/XP, Application Software’s: MS-Office, MS-Excel.

[Guest]

CURRICULUM-VITAE
AMIT SINGH RAWAT
E –mail : rawatamit407 AT gmail.com

CAREER OBJECTIVE :
I am searching for a job where I can get a position with a high degree of responsibility and an opportunity where I can enhance my skills & knowledge as well as to contribute towards the growth of the organization.
EDUCATION QUALIFICATIONS :
10th Passed from U.P Board.
12th Passed from U.P Board.
WORKING EXPERIENCE :
Working as Lab Technician at The Centre for Genomic Application, Okhla Industrial Estate Phase III, New Delhi, India since 1st January 2005 till date.
WORKING PROFILE :
- Working in Proteomics, Sequencing ,Genotyping, Oligosynthesis Department
- Experience on instrument like LC-MS, Nanodispenser, Beckman, Millipore, Sequenom Autoflex etc.
- Well experienced in gel electrophoresis like SDS-PAGE, Agarose Gel, PAGE for purification and quality check of single strand DNA and Proteins and other lab techniques like DNA isolation, Purification of DNA using enzymatic method, Post sequencing purification using Ethanol and Cleanseq methods.
- Preparation of buffers of different concentration like Tris buffer, SDS running buffer, Agarose gel running buffer and dilution of chemicals and buffers
- 2D Clean up Protein Sample & TCA-DOC precipitation a etc
- Record maintenance of Lab inventory for all general lab items and application specific reagents.
COMPUTATIONAL KNOWLEDGE :
Operating systems[]: Windows 98, XP. MS. Word, MS.Excel, MS Power point, Internet.

[Guest]

I, B.S.Yadav, would like to submitt my CV for the plant tissue culture position in your organisation.
Curriculum Vitae
Name :Badam Singh Yadav
Email :byadav AT hotmail.com
bsyadav60 AT gmail.com
ACADEMIC QUALIFICATIONS :
Degree University/Board Year Division Subjects
M.Sc (MS)
B. Ed. Sikkim Manipal University
Kanpur University
2006
1984 2nd
Theory-2nd Practical-1st - Ecology and Environment
- Mathematics & Science.
B.Sc. (BS) Kanpur University 1981 2nd Zoology, Botany & Chemistry
Intermediate (XII) U.P. Board 1979 2nd Biology, Chemistry, Physics Hindi & English.
High School (X) U.P. Board 1977 1st English, Science, Biology, Mathematics & Hindi.
Special Training :
- Department of Biotechnology (DBT), Government of India sponsored short term Technician Training Course on "Experiments in Genetic Engineering" at Department of Biotechnology, Madurai Kamraj University, Madurai, India-21st December, 1992 to 20th March, 1993.
EMPLOYMENT DETAILS :
Period Position held Institute/University
Jan Ist,2005 – till continue Senior Research Assistant Centre for Genetic Manipulationa and Crop Plants (CGMCP), Department of Genetics, Benito Jurez Marg, University of Delhi, South Campus, New Delhi.
Oct 31st 2003 – Aug.31st 2004 Research Assistant Dept. of Biological Sciences, Purdue University, West
Lafayette IN 47906 U.S.A.
July 22nd 2003 – Oct 30th 2003 Research Associate Plant Transformation Group, ICGEB,
Aruna Asaf Ali Marg, New Delhi-110067
May 31st 2002 – May 11th 2003 Research Assistant Dept. of Biological Sciences, Purdue University, West
Lafayette IN 47906 U.S.A.
Apr 3rd 2001 - May 30th 2002 Technical Research Assistant Dept. of Horticulture and Landscape Architecture, Purdue University, West Lafayette IN 47906 U.S.A.
May 12th 2000 - Mar 30th 2001 Technical Research Assistant Dept. of Biological Sciences, Purdue University, West
Lafayette IN 47906 U.S.A.
Feb. 3rd 1997 - May 11th 2000 Technical Assistant Plant Molecular Biology group, ICGEB,
Aruna Asaf Ali Marg, New Delhi-110067
Aug 31st 1993 - Sep 30th 1996 Research Assistant Biotechnology Division, Tata Energy Research Institute (TERI), New Delhi-110003
Dec 16th 1986 – Aug 30th 1993 Technical Assistant Biotechnology Division, Tata Energy Research Institute(TERI) New Delhi-110003
Dec 15th 1982 – Dec 15th 1986 Technical Assistant School of Life Sciences, Jawaharlal Nehru University (JNU), New Delhi-110067
Total Work Experience : 24 years of research experience in biological sciences
EXPERIENCE SUMMARY :
At present I am working as a Senior Research Assistant at the Centre for Manipulation of Crop Plants (CGMCP), University of Delhi, South Campus, Benito Jaurej Marg, New Delhi with Prof. Deepak Pental. I am assisting in various projects for improvement of crop Brassica for oilseed quality and seed yield. I have been doing tissue PCR, Sourthern and GFP analysis for identification of marker free transgenic Brassica. I am using tchniques of PCR and AFLP for whole genome selection, for gene mapping and for calculating percentage hybridity in CMS Brassica hybrids. I have been working on microspore culture technique to generate doubled haploid plants for isolation of low glucosinolate plants in Indian mustard B. juncea.
In Dr. Gelvin’s Lab, I was involved in the screening for HAT (Hyper-susceptible to Agrobacterium Transformation) mutants of Arabidopsis thaliana activation T-DNA tagged lines obtained from ABRC. Activation T-DNA tagging vector contains multimerized CaMV 35S preceding the right border. T-DNA integration into the plant chromosomes may cause transcriptional activation of downstream gene leading to dominant phenotype. I was screening activation T-DNA library using either a tumorigenic Agrobacterium strain or disarmed Agrobacterium strain with reporter gene (Gus gene with an intron) as a part of the T-DNA. In my screening till date I have identified several lines which show increased transformation efficiency in comparison to the wild type (HAT mutants). Further molecular characterization to identify the cause of the HAT phenotype is in progress.
Mysore et al has reported a T-DNA insertion in the RAT 5 (H2A1) gene which confers a RAT phenotype in the Agrobacterium mediated root transformation assays. The RAT phenotype can be complemented by the over-expression (CaMV promoter driven) of not only H2A1 but also by several others H2A member cDNAs. Over-expression of H2A1 cDNA in wild type plants causes an increase in the transformation efficiency in comparison to the wild type plant (Mysore et al). A recent report also shows differential expression of Histone genes in response to a transfer competent Agrobacterium infection of tobacco BY2 cells (Veena et al, 2003). With these observations, we are investigating whether the over-expression of other histone cDNAs in wild type plants affect the Agrobacterium mediated transformation efficiencies of the derived transgenic plants.
In Dr. Murphy’s Lab, I was involved in the construction of promoter : reporter plasmids using GUS or GFP as the reporter of interest. I had modified the existing plasmids containing the reporters of interest to allow them to be under the transcriptional control of the promoter of interest. I had transformed plants with these promoter: reporter plasmids and had screened and analyzed the transformed plants. I was also involved in the screening of the EMS mutagenized seed lines for mutants of the gene of interest. The mutants that can not perform a specific aminopeptidase reaction do not stain magenta in the presence of napthylopthalmic acid (NPA) and these mutants were then analyzed to identify the mutated gene.
While working in the Department of Biology with Prof. Stanton B. Gelvin earlier, I was involved in the screening for rat (Resistant to Agrobacterium Transformation) mutants of Arabidopsis thaliana T-DNA tagged lines obtained form INRA / Versailles and Dr. Ray Bressan of Purdue University. There are 3900 (INRA) and 6000 (Dr. Ray Bressan) independent T-DNA tagged lines respectively which I then screened by root transformation assay using Agrobacterium strains carrying either tumorigenic Ti plasmid or the disarmed strain carrying an antibiotic resistance gene (Kanamycin and Phosphinothrycin) on a binary vector. Our interest was to identify line that are resistant to tumor formation (for tumorigenic Agrobacterium strain) and inability to generate anti-biotic calli (for Agrobacterium strains carrying the corresponding antibiotic gene on a binary vector. Once such lines were identified, I performed genomic or cDNA complementation to restore the wild phenotype. These assays are useful in identifying potential plant genes involved in the Agrobacterium mediated transformation process.
I was also involved in the screening of the above mentioned T-DNA tagged lines to identify Arabidopsis thaliana plants disrupted in specific chromatin genes such as Histone Deacetyl Transferases (HDACs), Histone Acetyl Transferases (HATs). We employed a “Reverse genetics PCR based approach” for this process. This was done using gene specific primers for PCR of the T-DNA tagged planT-DNA. Amplification products indicated the T-DNA integration into or near the ORF of the gene of interest. Further confirmation of T-DNA integration was performed with southern blotting.
In I.C.G.E.B., I was involved in the identification and characterization of protein kinase C in Pisum sativum. Using Topoisomerase I as a substrate, the PKC type activity has been identified in the pea nuclear extract. This was confirmed by using various specific modulators and inhibitors of PKC from animal system as reference. We could partially purify the PKC type protein from the pea nuclear extract. To execute the above problem we used different approaches like affinity chromatography, gel permeation chromatography etc. Final analysis of protein was done by SDS PAGE using CBB (Coomassie Brilliant Blue) and silver staining.
I have expertise in protein kinase assay and protein phosphorylation. I have done amino acid analysis for characterization of phosphorylation of Topo I by PKC type activity in pea. The phosphorylated Topo I (by PKC) was immuno-precipitated by Topo I antibodies and further analyzed by Western with different antibodies for phospho-serine, phospho-threonine and phospho-tyrosine. I have a very good hand in RNA isolation and northern hybridization, which I have done for the determination of transcript level of Topoisomerase II in embryos, internodes, roots, leaves, callus cultures in dark and light conditions. Besides this, I have done northern for the determination of PCNA and Nucleolin level, in dark and light grown pea tissues. I have also worked on Vacuolar ATPaseB subunit in Tobacco. We cloned the gene from Tobacco (Accession no.AF220611) by screening with an EST (Accession no. AI920280) which was isolated by us, earlier. As the Vacuolar ATPases are involved in salt stress homeostasis, I also attempted to raise the transgenic of vacuolar ATPase in tobacco and to study the expression pattern of this gene under salinity stress. Besides these, I also studied the interaction of vacuolar ATPase with calcium binding protein (like calnexin and EhCaBP, a novel calcium binding protein from Entameoba histolytica) whose role in salinity stress was also shown in transgenic plants. Furthermore, I was also involved in the detailed molecular analysis of EhCaBP and Glyoxalase transgenic tobacco. I also tried to introduce EhCaBP and Glyoxalase I is economically important crops like rice, potato and Brassica. My other project was to establish and develop the in vitro pea culture system including suspension and single cell culture for understanding the cell-cycle control.
Earlier, I was involved in the cloning and characterization of pea ftsZ. In this project, I sequenced the whole 1.6 >kb gene and several other clones like putative cam-kinase, Phospholipase-D and Calcium Binding Protein from different plant systems.
At TERI, I have worked on research projects aimed at improving the yield and quality of Brassica crop, using both conventional and modern biotechnological approaches.
The projects involved work on :
At the lab level :
A. Development of efficient protocols for the production of transgenics through both protoplast-plasmid and explant-Agrobacterium interactions. I was associated with development of transgenic Brassica oleracea, Brassica nigra, Brassica campestris and Brassica juncea containing different antibiotic and herbicide resistance markers, and maintenance of these transgenics under containment conditions in the net house.
B. Production of somatic hybrids with the aim of
a. introgression of desirable traits from related alien species for imparting disease resistance, heat tolerance etc.
b. for improving and/or rectifying cytoplasmic male sterility induced abnormalities like chlorosis or drastic floral deformities by replacement of chloroplasts and recombination of mitochondrial genomes.
c. molecular characterization of the hybrids for their organelle composition and transgenics for characterizing the pattern of alien gene integration.
At field level :
A. Maintenance of germplasm
B. Pollination's for generating intergeneric, interspecific and intervarietal crosses
C. Recording field data related to assessment of yield parameters in heterosis breeding programme.
Technical Expertise :
- Recombinant DNA Techniques
Plasmid DNA isolation : mini- & maxi-prep
Genomic DNA isolation from higher plants
Restriction digestion, ligation, transformation
Agarose gel electrophoresis of nucleic acids
Southern, Northern and Colony hybridizations
Rescue T-DNA/Plant DNA junctions
Over-expression of cDNA clones in various plant system
Polymerase Chain Reaction
Single Primer PCR
Tail PCR
RT PCR
Inverse PCR
TissuePCR
Adapter ligation binding PCR (Genome walker)
Total RNA isolation from higher plants
Screening of cDNA library for specific clone
Sequencing (Sanger Dideoxy chain termination method)
Kinase Assay
Phospho amino/acid Analysis
Immuno-precipitation
Assay of Topoisomerase I
Protein purification techniques (Affinity chromatography and gel permeation)
SDS-PAGE
Over-expression of clone (s) in E. coli
Western blot analysis
In vivo phosphorylation
In gel kinase assay
AFLP
- Plant Tissue Culture Techniques
Preparation of media
Micropropagation
PEG mediated direct DNA uptake technique
Agrobacterium mediated transformation of plants
Histochemical GUS assay
Analysis of transgenic plants
Maintenance of hybrid and other genetic stock culture
Protoplast isolation
Protoplast fusion
Protoplast culture
Protoplast regeneration
Generation of cell lines
Microspore isolation, culture and regeneration of shoots
- Microbiology Techniques
Maintenance of microbial cultures
Bacterial conjugation
Maintenance of yeast master culture and mutants (rho+ and rho-)
Serial dilution technique
Harvesting and lyophilization of yeast cells.
- Hands on experience with software like Mac-Vector, DNA Strider, Adobe Photoshop, MS-Word, PowerPoint , Internet etc
SYMPOSIA/ CONFERENCES ATTENDED :
- 25th Crown Gall Conference, August 13-17, 2004, University of Illinois, Urbana-Champaign
- 24th Crown Gall Conference, November 21st – 23rd 2004, Cornell University, Ithaca, NY.
- Purdue University Genomics Symposium, October 12-13 2002, Purdue University, West Lafayette, IN, USA.
- 23rd Crown Gall Conference, November 1-3 2002, University of Minnesota, Minneapolis, MN, USA
- First ICGEB Symposium on Plant Signal Transduction, October 4 - 6 1999, New Delhi – India
-
POSTER PRESENTED :
- Identification of Arabidopsis mutants that are hyper-susceptible to Agrobacterium transformation (hat mutants) Badam S. Yadav, Joerg Spantzel, Karen M, Oskins, Alameri, Esan Wilkinson and Stanton B. Gelvin, Department of Biological Sciences, Purdue University,
West Lafayette, IN 47907-1392. 25th Crown Gall Conference, University of Illinois at Urbana-Champaign, Illinois USA
PUBLICATIONS :
1. Mukhopadhyay, A, Arumugam, N Pradhan, AK, Murthi, HN, Yadav BS, Sodhi, YS, and Pental D (1994). Somatic hybrid with substitution type configuration TCBB for the transfer of nuclear and organelle genes from Brassica tournefortii TT to allotetraploid oil seed crop Brassica carinata BBCC. Theor. Appl. Genet. 89 : 19-25.
2. Pradhan, AK, Arumugam, N, Mukhopadhyay, A, Gupta, V, Yadav, B.S. and Verma, JK (1994). Development of improved cytoplasmic male sterile lines in Brassica through somatic cell hybridization. Proceeding International Rapeseed Congress, 4-7 July, Cambridge, U.K.
3. Hop DV, Gaikwad AS, Yadav BS, Reddy MK, Sopory SK and Mukherjee SK. Pea PCNA suppresses DNA Topoisomerase I and its transcription is light stimulated. Plant Journal. 1999 19(2), 153-162.
4. Reddy MK, Nair S, Tewari KK Mudgil Y, Yadav BS and Sopory SK. Cloning and characterization of a cDNA Encoding Topoisomerase II in Pea and analysis of its expression in relation to cell proliferation. Plant Molecular Biology. 1999 41(1) 125-137).
5. Yanmin Zhu, Jaesung Nam, Jaime M. Humara, Kirankumar S. Mysore, Lan-Ying Lee, Hongbin Cao, Lisa Valentine, Jingling Li, Anthony D. Kaiser, Andrea L. Kopecky, Hau-Hsuan Hwang, Saikat Bhattacharjee, Praveen K. Rao, Tzvi Tzfira, Jyothi Rajagopal, HoChul Yi, Veena, Badam S. Yadav, Yan M. Crane, Kui Lin, Yves Larcher, Matthew J.K. Gelvin, Marnie Knue, Cynthia Ramos, Xiaowen Zhao, Susan J. Davis, Sang-Ic Kim, C.T. Ranjith-Kumar, Yoo-Jin Choi, Vipin K. Hallan, Sudip Chattopadhyay, Xiangzhen Sui, Alicja Ziemienowicz, Ann G. Matthysse, Vitaly Citovsky, Barbara Hohn, and Stanton B. Gelvin, Identification of Arabidopsis rat Mutants, Plant Physiol. 132 : 494-505
6. Narendra Tuteja, Malireddy Kodandarami Reddy, Yashwanti Mudgil, Badam Singh Yadav, Meena Rani Chandok, and Sudhir Kumar Sopory, Pea DNA Topoisomerase I Is Phosphorylated and Stimulated by Casein Kinase 2 and Protein Kinase C, Plant Physiol. 132 : 2108-2115; First published on July 10, 2003; 10.1104/pp.103.024273
Accession Numbers Acquired
1. Gaikwad AS, Mukherjee SK, Babberwal V, Yadav BS and Tiwari KK Pisum sativum mRNA for ftsZ gene. (Genbank Accession number : Y15383).
2. Pandey GK, Sopory SK and Yadav BS. DD5.2e Nicotiana tabacum cv. xanthi differential display Nicotiana tabacum cDNA clones DD5.2e 3' similar to vacuolar ATPase, B subunit. (genbank accession number : AI920280)
3. Pandey GK, Sopory SK and Yadav BS. Bst10.1 mannitol Stress cDNA lib of Brassica juncea CV Pusa bold Brassica juncea cDNA clone Bst10.1 3'. (genbank accession number : AI931183)
4. Pandey GK, Sopory SK and Yadav BS. DD5.2e Nicotiana tabacum cv. Xanthi differential display Nicotiana tabacum cDNA clones DD5.2e 3' similar to Polyprotein. (genbank accession number : AI931182).
5. Pandey, GK, Yadav, BS, Reddy, MK and Sopory, SK Nicotiana tabacum vacuolar H+-ATPase B subunit mRNA, complete cds. (Genebank accession number AF220611).
6. Pandey, GK, Pandey, A, Sopory, SK and Yadav, BS 12.1t3 Mannitol stress inducible cDNA lib. Of Brassica juncea cDNA clone 12.1t3 3' similar to Arginine decarboxylase, mRNA sequence (genebank accession number AW288082).
7. Pandey, GK, Pandey, A, Sopory, SK and Yadav, B.S. 12.1T7 Mannitol Stress inducible cDNA library of Brassica juncea cv pusa bold Brassica juncea cDNA clone 12.1T7 3' similar to Arginine decarboxylase, mRNA sequence (AW288083).
8. Pandey, A, Sopory, SK and Yadav, BS DD2.1 Nicotiana tabacum cv. Xanthi differential display Nicotiana tabacum cDNA clone DD2.1e 5' similar to polyprotein, mRNAsequence (AW288081).
9. Reddy, MK, Nair, S, Tewari, KK, Mudgil, Y, Yadav, BS and Sopory, SK Pisum sativum mRNA for non-specific lipid transfer protein. (genebank accession number Y14560).
10. Reddy, MK, Nair, S, Tewari, KK, Mudgil, Y, Yadav, BS and Sopory, SK Pisum sativum mRNA for ribonucleoprotein (genebank accession number Y14557).
11. Reddy, MK, Nair, S, Tewari, KK, Mudgil, Y, Yadav, BS and Sopory, SK Pisum sativum topoisomerase II gene, promoter region and partial cds. (gnenebank accession number AF 144649).
12. Reddy, MK, Nair, S, Tewari, KK, Mudgil, Y, Yadav, BS and Sopory, SK Pisum sativum mRNA for topoisomerase II (genebank accession number Y14559).

[Guest]

SHOWKAT RASHID WANI
E-MAIL ID :SHOWKAT560 AT YAHOO.CO.IN
SUMMARY
- Postgraduate in Biotechnology from H.N.B Garhwal university Srinagar GarHWAL UTTRAKHAND
- Advanced post(M.Sc) diploma from Aligarh Muslim University ALIGARH (U.P)
CAREER OBJECTIVE
Seeking position in an organization where I can apply my thoughts and skills for the development of the organization as well as my personal growth.
Area of interest.
- Production of planting material of different crops problems with the help of different tissue culture techniques.
- Initiation of newer crops for tissue culture.
- Developing protocols for reducing mortality and Contamination.
- Develop method to reduce existing cost of tissue Culture plantlets.
- Microbiological techniques.
- Biotechnological techniques.
ACADEMIC DETAILS
- M.Sc biotechnology from H.N.B Garhwal university uttrakhand with 63% marks in year 2007
- Advanced post(M .SC) diploma from AMU Aligarh sponsored by department of Biotechnology New Delhi.
- B.Sc from univesity of kashmir with 49% in year 2004
- Certificate course in Clinical trail,research and data management from Bioinformatics institute of india Noida (U.P)
Experience :
Have done six months research project titled “effect of TDZ on in vitro regeneration of leaucaena leucocephala (Lam.) de wit” through various explants” from AMU Aligarh.
ADDITIONAL SKILLS
Computer Skills :
Knowledge of MS Office and Internet operations.
Techniques Known :
- Plant tissue culture :(Micropropogation,callus culture, synthetic seed Protoplast isolation culture,
- DNA Isolation Methods (Plant, Bacteria )
- Restriction Digestion Methods

[Unregistered]

RESUME
Personal details :-
Name : Mr. Hemant Yadav
Father’s name : Mr. Rishi Pal Singh
Date of birth : 05th July, 1992
Marital Status : Unmarried
Nationality : Indian

Bio : simple but dashing in nature. I m serious and punctual about my work.

Educational Details :-
Passed 10th class from UP Board in 2007.
Passed 12th class from UP Board in 2009.
Passed Graduation (B.Sc Zoology, Botony, Chemistry) from M.J.P.Rohilkhand University Bareilly in 2012.
appearing P.G. Diploma in Biotechnology (Tissue Culture) Waiting Result from Board of technical education Lucknow.

Extra Qualification :-
NCC C Certificate.
One month training on Dayal Seed Pvt Ltd Partapur Meerut.
2 Days Project on Cesh.

Pledge :-
The above information given is perfectly true. Make sure that I will do my work with full honesty, punctuality, and whole heartedly. I will not give any chance that anyone lifts finger on me and my work.

Thanking you for seeing my resume.

Place :Bahjoi

Signature:-
HEMANT YADAV