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[QUOTE=hemalathaijt;192496][B]Document Described : Alagappa University
DISTANCE EDUCATION
M.Phil. (Bio-Technology) DEGREE EXAMINATION,
MAY 2011.
Non-Semester
RESEARCH METHODOLOGY AND LABORATORY TECHNIQUES
(2006 Batch)[/B]
[B]Time[/B] : Three hours [B]Maximum [/B]: 100 marks

[URL]http://www.alagappauniversity.ac.in/examination/model_question_paper_details.php?paper_month_id=9&college_id=2[/URL]
[URL="http://www.alagappauniversity.ac.in/files/question_paper/1-M.Phil%20%28Bio-Techn.%29.doc"]http://www.alagappauniversity.ac.in/files/question_paper/1-M.Phil%20(Bio-Techn.).doc[/URL]

Answer any FIVE questions.
(5 ? 20 = 100)
All questions carry equal marks.

1.Write about the principles and applications of SEM and TEM.

2.Describe the principle of Gas chromatography and what are the differences between Gas and HPLC.

3.Write note on ELISA and Immunoelectron microscopy.

4.Explain in detail about Isoelectrofocusing and application of immunoelectrophoresis.

5.Describe the principle of Southern Blotting technique. Differences between Northern and Western blotting techniques.

6.Write note on PCR principle. Explain PCR based DNA fingerprinting techniques.

7.Give an account on protein primary and secondary structure prediction. Explain protemic tools analysis at EXPASY Server.

8.Describe the primer designing tools in genomics, prediction of genes, promoters  and splice donor and acceptor sites.[/QUOTE]

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