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Biocon Ltd Bangalore : Scientist cell culture
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[QUOTE=Guest;142360]To Whomsoever It May Concern I am writing you regarding potential employment opportunities. I am seeking a microbiology-related position in an R&D setting or a university, where my skills can be utilized effectively. I hold a Masters in Microbiology from Mangalore University in India. The research experience I have acquired is in microbiological, molecular and biochemical fields. The resume below will give you a brief synopsis of my academic/work experience history in the microbiological field. I would appreciate being considered for any position with a university laboratory or a research-driven biotech/food company that you feel match my skills and would add value to you. Should you wish to contact me, please use my contact information below. I am looking forward to hearing from you. CURRICULUM VITAE JASHMI .K.C. [b]E-Mail [/b]: jashmineethu AT gmail.com [b]OBJECTIVE [/b]: My objective is to pursue a scientific career in one of the many microbiological areas within a research-oriented biotech/food industry or in the academia. I would like to join a team where my skills can be utilized effectively in a setting that encourages personal and professional growth. [b]EDUCATIONAL QUALIFICATION [/b]: Exam passed School/college Year % MARKS M.Sc (Microbiology) Mangalore University 2009 74% (Aggregate of 4 semesters ) B.Sc (Chemistry,Botany, Zoology ) Mangalore University 2007 71% [b]THEORITICAL KNOWLEDGE [/b]: General microbiology, Biochemistry, Mycology/Phycology, Microbial diversity, Microbial physiology, Biostatistics/ Bioinformatics, Biochemical technique, Genetics, Molecular biology, Plant Pathology, Industrial microbiology, Immunology and Medical microbiology, agricultural microbiology, Environmental microbiology, Food and dairy microbiology and Microbial Biotechnology [b]PRACTICAL KNOWLEDGE [/b]: - Microscopy, microbial cultures and samples preparation - Growth, Isolation and identification of microorganisms - Bacterial staining (such as Gram staining, negative staining, endospore staining, simple staining) - Microbial isolation techniques (such as culture media, single colony isolation, pour plate technique, streak plate technique, direct plating etc.) - Microbial assay of antibiotics by disc diffusion method [b]- Laboratory testing and analysis [/b]: UV spectrophotometric analysis, chromatography analysis (Thin layer chromatography, Column chromatography, Two dimensional chromatography, paper chromatography) electrophoresis, operating water analyzer, Dot ELISA. Hanging drop technique, micrometry, Hemocytometer, Radial Immunodiffusion assay by Mancini method, Ouchterlony Double Diffusion assay, Rocket ImmunoElectrophoresis Assay, SDS-PAGE, Buffer preparation, Precipitation of protein , Seed health testing , Estimation of RNA by Orcinol method, Estimation of DNA by DPA method, Extaction of DNA from yeast , Isolation of plasmid DNA, Transformation and Transduction in bacteria, Agarose Gel Electrophoresis. [b]MASTER THESIS [/b]: Worked on “Isolation of coliforms from drinking water sources from the villages of madikeri taluk”. The project was carried out with the aim of creating awareness about spreading diseases through water in the villages. The water samples were collected and tested for physicochemical and microbiological parameters. The physicochemical tests included color, odor, taste, temperature, PH, acidity, alkalinity, TDS chloride, sulphate, salinity, conductivity, calcium, CO2, hardness, magnesium, Biological oxygen demand, dissolved and Chemical oxygen demand. The microbiological tests included the analysis for Coliforms. The results showed that Enterobacter Sp., Escherichia coli and Klebsiella were the common microorganisms higher than all the time alone will have higher than that there were Among the isolates obtained from water samples. Among them, Enterobacter showed higher (50%) contamination when compared to E Coli. and Klebsiella sp. Both physiochemical and microbial quality of majority of water samples were in the range of standard laboratory value and thus considered to be safe for drinking purposes. However, few samples showed the presence of coliforms indicating the water as impotable for drinking purposes. In some samples, although physicochemical parameters were in permissible level, it showed the presence of pathogenic coliforms. The significance of the present work was helpful in creating awareness regarding the poor quality of water sources in these villages. [b]EXPERIENCE [/b]: Worked as a chemist/microbiologist in a Calypso Food Pvt. Ltd., Hassan, for 1 month duration. [b]Theme Area I [/b]: Diversity analysis Documentation and conservation of cultures of Colletotrichum isolates 100 isolates of Colletotrichum have been characterized based on morphological characters and preserved under lab conditions Pathogenicity trials with distinct isolates on differentials Five black pepper isolates and five isolates from cardamoms which were found to be aggressive were short listed for pathogenicity trial. The black pepper isolates were inoculated on 12 black pepper varieties/ cultivars to study the differential reaction [b]Theme Area II [/b]: Diagnostics Standardization and validation of DNA isolation protocols from crop debris and host tissues [b]Theme Area III [/b]: Epidemiology Studying disease initiation and spread in relation to weather parameters Influence of weather parameters such as temperature and rainfall on the incidence of anthracnose in black pepper has been studied. The results indicated that, the disease was initiated during second week of May in the experimental farm, Appangala and attained the peak during August. The disease initiation and subsequent spread was favoured by rainfall pattern and number of rainy days [b]Theme Area IV [/b]: Genomics Development of markers for virulence and fungicide sensitivity Five black pepper isolates and five isolates for cardamom which were found to be aggressive were short listed for this study. Three black pepper isolates were collected from three plantations in Kodagu where the fungicidal sprays are taken up regularly and being evaluated for fungicidal sensitivity. [b]Theme Area IV [/b]: Host resistance Screening of germplasm Two cardamom accessions (IC – 349613, IC - 349588) exhibited highly resistant reaction against leaf blight of cardamom IDM strategies Standardization of mass multiplication methods using local agro-wastes Laying out field trials with potential biocontrol agents, fungicides and plant extracts on susceptible and resistant selection Three fungicides, one plant extract and one Trichoderma spp. were short listed for laying out field trials [b]SUMMER PROJECT [/b]: Worked on the project entitled “Study on yeast diversity in food processing Industry”. I included myself in the role to maintain documentation of the laboratory as well as to maintain quality of the food product. [b]HANDLING PATHOGEN IN LAB [/b]: E.coli, shigella, coliform, V. cholera ,Klebsiella, Pseudomonas, Streptococcus, Enterobacter, Salmonella, Coletrotrichum, Trichoderma, Aspergillus,Penicilium, Gleocladium, Mucor, Rhizopus, etc. [b]SAMPLE HANDLED [/b]: [b]Water [/b]: borewell, tap water, well water, pond water; Soil, milk, blood, plant extract for their antimicrobial activity, different spices and food preservatives. COMPUTER KNOWLEDGE Diploma in computer application with Tally. [b]CERTIFICATE [/b]: * Participate in Awareness training program on Biodiversity Related issues and people biodiversity registers on August 28th 2007 In dept of microbiology, Madikeri. * National seminar certificate on forest and Microbial diversity and its Relevance on 26-30 December 2007 In dept of microbiology, Madikeri. * Indian Academy of sciences outreach seminar workshop on Molecular biology on 11th and 12th of April 2008, in dept of Biochemistry, Madikeri.[/QUOTE]
